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PMID: 808630 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The kinetics of resealing of washed erythrocyte ghosts.

The Journal of membrane biology ·Vol. 22 ·No. 3-4 ·1975-07-24 ·Pages 231-53

Johnson RM

Abstract

The kinetics of resealing, defined as the recovery of impermeability to macromolecules, of well-washed human erythrocyte ghosts has been determined. The resealing process is first-order at temperatures above 20-25 degrees C in isotonic salt, with rate constants ranging from 0.01-0.15 min-1. Below 20 degrees C, resealing occurs, but a long lag period is observed. Other erythrocyte membrane properties suggest a transition at about 20 degrees C, and it is possible that resealing rates are a measure of membrane fluidity. A temperature-induced reduction of resealed ghost volume was also observed. The effect of ionic strength on resealing parameters was determined. Low ionic strength buffers prevent resealing, which is also consistent with resealing as a lipid-related event. The effect of microtubule disrupting drugs and changes in the method of preparing ghosts are also described.

MeSH Terms
Cell Membrane/analysis,physiology Cell Membrane Permeability Colchicine/pharmacology Edetic Acid/pharmacology Erythrocytes/analysis,physiology,ultrastructure Hemolysis Humans Kinetics Osmolar Concentration Sodium Chloride/pharmacology Specimen Handling Sucrose/pharmacology Temperature Vinblastine/pharmacology
Chemicals
Sodium Chloride Sucrose Vinblastine Edetic Acid Colchicine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Johnson R M
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41 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01868173
Published
1975-07-24
Pages
231-53
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01868173 View journal details
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