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PMID: 6887347 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Closed circular viral DNA and asymmetrical heterogeneous forms in livers from animals infected with ground squirrel hepatitis virus.

Journal of virology ·Vol. 48 ·No. 1 ·1983-10-00 ·Pages 1-9

Weiser B, Ganem D, Seeger C, Varmus HE

Abstract

To identify possible intermediates in the replication of ground squirrel hepatitis virus, we characterized the major forms of intracellular virus-specific DNA in the livers of infected ground squirrels. A variety of DNA species were found: covalently closed circular molecules, relaxed circular molecules, and a heterogeneous collection of molecules that migrated ahead of closed circular DNA during agarose gel electrophoresis. The heterogeneous DNA was at least partly single stranded, consisted of minus strands in a greater than eight-fold mass excess of plus strands, and was tightly associated with protein.

MeSH Terms
Animals Centrifugation, Density Gradient DNA, Circular/analysis DNA, Single-Stranded/analysis DNA, Viral/analysis,metabolism Hepatitis Viruses/analysis Hepatitis, Viral, Animal/microbiology Liver/microbiology Nucleic Acid Conformation Proteins/metabolism Sciuridae/microbiology
Chemicals
DNA, Circular DNA, Single-Stranded DNA, Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Weiser B
Ganem D
Seeger C
Varmus H E
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.48.1.1-9.1983
Published
1983-10-00
Pages
1-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255316
Subset
IM
Grants
NCI NIH HHS · CA12705 · United States
NCI NIH HHS · CA19287 · United States
NHLBI NIH HHS · HL01048 · United States
External Links
PubMed source DOI: 10.1128/JVI.48.1.1-9.1983 PMC Full Text View journal details
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