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PMID: 6823008 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein covalently bound to minus-strand DNA intermediates of duck hepatitis B virus.

Journal of virology ·Vol. 45 ·No. 1 ·1983-01-00 ·Pages 165-72

Molnar-Kimber KL, Summers J, Taylor JM, Mason WS

Abstract

Analysis of duck hepatitis B viral DNA by gel electrophoresis, Southern blotting, and binding to benzoylated naphthoylated DEAE-cellulose showed that a protein is bound to the minus-strand virion DNA as well as to the full-length single strand, minus-strand species, and minus-strand DNA intermediates isolated from replicating complexes present in infected duck liver. By utilizing a modified dideoxynucleotidyl sequencing method, it was shown that the protein is covalently bound to the smallest detectable growing strands (ca. 30 bases) and that minus-strand synthesis begins at a unique site. These results support the notion that the protein may function as a primer for synthesis of the minus-strand DNA.

MeSH Terms
Animals Base Sequence DNA Replication DNA, Viral/metabolism Ducks Hepatitis B virus/metabolism Liver/microbiology Viral Proteins/metabolism Virus Replication
Chemicals
DNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Molnar-Kimber K L
Summers J
Taylor J M
Mason W S
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20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.45.1.165-172.1983
Published
1983-01-00
Pages
165-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256398
Subset
IM
Grants
NIAID NIH HHS · AI-15166-04 · United States
NCI NIH HHS · CA-09035-07 · United States
NCI NIH HHS · CA-22651-05 · United States
External Links
PubMed source DOI: 10.1128/JVI.45.1.165-172.1983 PMC Full Text View journal details
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