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PMID: 6835206 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Heat shock alters nuclear ribonucleoprotein assembly in Drosophila cells.

Molecular and cellular biology ·Vol. 3 ·No. 2 ·1983-02-00 ·Pages 161-71

Mayrand S, Pederson T

Abstract

Heterogeneous nuclear RNA is normally complexed with a specific set of proteins, forming ribonucleoprotein particles termed hnRNP. These particles are likely to be involved in mRNA processing. We have found that the structure of hnRNP is profoundly altered during the heat shock response in Drosophila cultured cells. Although hnRNA continues to be synthesized at a near-normal rate during heat shock, its assembly into hnRNP is incomplete, as evidenced by a greatly decreased protein content of the particles in Cs2SO4 density gradients. RNA-protein cross-linking conducted in vivo (Mayrand and Pederson, Proc. Natl. Acad. Sci. U.S.A. 78:2208-2212, 1981) also reveals that hnRNA made during heat shock is complexed with greatly reduced amounts of protein. The block of hnRNP assembly occurs immediately upon heat shock, even before the onset of heat shock protein synthesis. Additional experiments reveal that hnRNP assembled normally at 25 degrees C subsequently disassembles during heat shock. The capacity for normal hnRNP assembly is gradually restored after heat-shocked cells are returned to 25 degrees C. Heat-shocked mammalian cells also show a similar block in hnRNP assembly. We suggest that incomplete assembly of hnRNP during heat shock leads to abortive processing of most mRNA precursors and favors the processing or export (or both) of others whose pathway of nuclear maturation is less dependent on, or even independent of, normal hnRNP particle structure. This hypothesis is compatible with a large number of previous observations.

MeSH Terms
Animals Cells, Cultured Cross-Linking Reagents Drosophila Gene Expression Regulation Heterogeneous-Nuclear Ribonucleoproteins Hot Temperature Mice Nucleoproteins/genetics RNA Processing, Post-Transcriptional RNA, Heterogeneous Nuclear/biosynthesis Ribonucleoproteins/genetics
Chemicals
Cross-Linking Reagents Heterogeneous-Nuclear Ribonucleoproteins Nucleoproteins RNA, Heterogeneous Nuclear Ribonucleoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mayrand S
Pederson T
References (48)
48 references, click to expand
  1. Messenger RNA in heat-shocked Drosophila cells.
    J Mol Biol. 1977 Feb 5;109(4):559-87 PMID: 403287
  2. The metabolism of poly (A)+ and poly(A)-hnRNA in cultured Drosophila cells studied with a rapid uridine pulse-chase.
    Cell. 1977 May;11(1):105-13 PMID: 872216
  3. Transcription at two heat shock loci in Drosophila.
    Cell. 1977 Oct;12(2):441-51 PMID: 410501
  4. 5'-terminal structures of poly(A)+ cytoplasmic messenger RNA and of poly(A)+ and poly(A)- heterogeneous nuclear RNA of cells of the dipteran Drosophila melanogaster.
    J Mol Biol. 1978 Apr 25;120(4):487-515 PMID: 418182
  5. The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1047-51 PMID: 98262
  6. Nucleoprotein organization of inverted repeat DNA transcripts in heterogeneous nuclear RNA-ribonucleoprotein particles from HeLa cells.
    J Mol Biol. 1978 Jul 5;122(3):361-78 PMID: 691046
  7. Isolation and characterization of ribonucleoprotein particles containing heterogeneous nuclear RNA.
    Methods Cell Biol. 1978;17:377-99 PMID: 703621
  8. Sequence organization and transcription at two heat shock loci in Drosophila.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5613-7 PMID: 103099
  9. Evidence for a role of RNA in eukaryotic chromosome structure. Metabolically stable, small nuclear RNA species are covalently linked to chromosomal DNA in HeLa cells.
    J Mol Biol. 1979 Mar 15;128(4):451-80 PMID: 571474
  10. The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.
    Cell. 1979 Apr;16(4):807-14 PMID: 455450
  11. The induction of gene activity in drosophilia by heat shock.
    Cell. 1979 Jun;17(2):241-54 PMID: 110462
  12. Studies of cloned sequences from four Drosophila heat shock loci.
    Cell. 1979 Dec;18(4):1359-70 PMID: 117907
  13. Definition and mapping of adenovirus 2 nuclear transcription.
    Methods Enzymol. 1980;65(1):768-85 PMID: 6246373
  14. Varying patterns of protein synthesis in Drosophila during heat shock: implications for regulation.
    Dev Biol. 1980 Jun 15;77(2):463-79 PMID: 7399133
  15. Messenger RNA processing and nuclear structure: isolation of nuclear ribonucleoprotein particles containing beta-globin messenger RNA precursors.
    J Cell Biol. 1980 Oct;87(1):47-54 PMID: 6932401
  16. The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha.
    Nature. 1980 Oct 2;287(5781):401-8 PMID: 6159536
  17. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  18. Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5390-3 PMID: 6254079
  19. Transcription and metabolism of RNA from the Drosophila melanogaster heat shock puff site 93D.
    Chromosoma. 1980;80(3):237-52 PMID: 6160021
  20. Specific activation of puff 93D of Drosophila melanogaster by benzamide and the effect of benzamide treatment on the heat shock induced puffing activity.
    Chromosoma. 1980;81(1):125-36 PMID: 6160023
  21. Translational control of protein synthesis in response to heat shock in D. melanogaster cells.
    Cell. 1980 Dec;22(3):825-34 PMID: 6780199
  22. In vitro translation of Drosophila heat-shock and non--heat-shock mRNAs in heterologous and homologous cell-free systems.
    Cell. 1981 Feb;23(2):595-603 PMID: 6781760
  23. Regulated transcription of the genes for actin and heat-shock proteins in cultured Drosophila cells.
    J Cell Biol. 1981 Feb;88(2):323-8 PMID: 6162853
  24. The absence of introns within a human fibroblast interferon gene.
    Nucleic Acids Res. 1981 Jan 24;9(2):247-66 PMID: 6163136
  25. Evidence for a unique human fibroblast interferon (IFN-beta 1) chromosomal gene, devoid of intervening sequences.
    Nucleic Acids Res. 1981 Feb 11;9(3):461-71 PMID: 6164047
  26. Human fibroblast interferon gene lacks introns.
    Nucleic Acids Res. 1981 Mar 11;9(5):1045-52 PMID: 6164984
  27. Nuclear ribonucleoprotein particles probed in living cells.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2208-12 PMID: 6941279
  28. Sequence homologies in the 5' regions of four Drosophila heat-shock genes.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3775-8 PMID: 6791161
  29. Regulation of protein synthesis during heat shock.
    Nature. 1981 Sep 24;293(5830):311-4 PMID: 6792546
  30. Structure of nuclear ribonucleoprotein: identification of proteins in contact with poly(A)+ heterogeneous nuclear RNA in living HeLa cells.
    J Cell Biol. 1981 Aug;90(2):380-4 PMID: 6169730
  31. Base-pairing interactions between small nuclear RNAs and nuclear RNA precursors as revealed by psoralen cross-linking in vivo.
    Cell. 1981 Nov;26(3 Pt 1):363-70 PMID: 6173132
  32. Ribonucleoprotein organization of eukaryotic RNA. XV. Different nucleoprotein structures of globin messenger RNA sequences in nuclear and polyribosomal ribonucleoprotein particles.
    J Mol Biol. 1981 Aug 25;150(4):509-24 PMID: 7328643
  33. Factors involved in the expression of gene activity in polytene chromosomes.
    Chromosoma. 1968;24(4):418-37 PMID: 5708273
  34. Regulation of protein synthesis in HeLa cells: translation at elevated temperatures.
    J Mol Biol. 1969 Jan;39(2):315-33 PMID: 5362672
  35. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  36. Biogenesis and characterization of histone messenger RNA in HeLa cells.
    J Mol Biol. 1972 Jun 28;67(3):397-406 PMID: 5045304
  37. Regulation of protein synthesis in mammalian cells. V. Further studies on the effect of actinomycin D on translation control in HeLa cells.
    J Mol Biol. 1973 Oct 25;80(2):243-54 PMID: 4763984
  38. Proteins associated with heterogeneous nuclear RNA in eukaryotic cells.
    J Mol Biol. 1974 Feb 25;83(2):163-83 PMID: 4821869
  39. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  40. Analysis of drosophila mRNA by in situ hybridization: sequences transcribed in normal and heat shocked cultured cells.
    Cell. 1975 Apr;4(4):395-404 PMID: 1122559
  41. hnRNA size and processing as related to different DNA content in two dipterans: Drosophila and Aedes.
    Cell. 1975 Jul;5(3):281-90 PMID: 807333
  42. Comparison of proteins bound to heterogeneous nuclear RNA and messenger RNA in HeLa cells.
    J Mol Biol. 1975 Aug 15;96(3):353-65 PMID: 1165583
  43. The effect of heat shock on RNA synthesis in Drosophila tissues.
    Cell. 1976 May;8(1):43-50 PMID: 954091
  44. Small nuclear RNA U2 is base-paired to heterogeneous nuclear RNA.
    Science. 1982 Jul 30;217(4558):456-8 PMID: 6178162
  45. Small nuclear ribonucleoproteins of Drosophila: identification of U1 RNA-associated proteins and their behavior during heat shock.
    Mol Cell Biol. 1982 Aug;2(8):914-20 PMID: 6182453
  46. Interferon production by different viruses.
    Virology. 1963 Jan;19:8-12 PMID: 13975488
  47. STUDIES ON ISOLATED NUCLEI. II. ISOLATION AND CHEMICAL CHARACTERIZATION OF NUCLEOLAR AND NUCLEOPLASMIC SUBFRACTIONS.
    J Cell Biol. 1963 Aug;18:293-312 PMID: 14079490
  48. CIRCULATING INTERFERON AFTER MEASLES VACCINATION.
    N Engl J Med. 1965 Jul 22;273:198-201 PMID: 14306336
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.2.161-171.1983
Published
1983-02-00
Pages
161-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368518
Subset
IM
Grants
NIGMS NIH HHS · GM 28274 · United States
External Links
PubMed source DOI: 10.1128/mcb.3.2.161-171.1983 PMC Full Text View journal details
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