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PMID: 6807285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Active sites of beta-lactamases. The chromosomal beta-lactamases of Pseudomonas aeruginosa and Escherichia coli.

The Biochemical journal ·Vol. 201 ·No. 3 ·1982-03-01 ·Pages 621-7

Knott-Hunziker V, Petursson S, Jayatilake GS, Waley SG, Jaurin B, Grundström T

Abstract

An acyl-enzyme was isolated from certain chromosomal beta-lactamases and a penicillin. The penicillin was cloxacillin which, although it is a substrate for these enzymes, has such a low kcat. that it functions as an inhibitor. The enzymes were from the mutant of Pseudomonas aeruginosa 18 S that produces the beta-lactamase constitutively [Flett, Curtis & Richmond (1976) J. Bacteriol. 127, 1585-1586; Berks, Redhead & Abraham (1982) J. Gen. Microbiol., in the press] and from Escherichia coli K-12 (the ampC beta-lactamase) [Boman, Nordström & Normak (1974) Ann. N.Y. Acad. Sci. 235, 569-586]. The acyl-enzymes have been degraded to determine the residue labelled, and the sequence around it. The residue labelled is serine. The sequences around the labelled serine in these two beta-lactamases are exceedingly similar. However, the sequences are quite different from those around the active site serine in the beta-lactamases previously studied. There is thus more than one class of serine beta-lactamases.

MeSH Terms
Amino Acid Sequence Binding Sites Chromatography, High Pressure Liquid Chromosomes, Bacterial/enzymology Cloxacillin/metabolism Escherichia coli/enzymology Peptide Fragments/isolation & purification Pseudomonas aeruginosa/enzymology beta-Lactamases/classification,metabolism
Chemicals
Peptide Fragments beta-Lactamases Cloxacillin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Knott-Hunziker V
Petursson S
Jayatilake G S
Waley S G
Jaurin B
Grundström T
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27 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2010621
Published
1982-03-01
Pages
621-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1163689
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2010621 PMC Full Text View journal details
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