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PMID: 6799741 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A mutation in the RNA polymerase beta' subunit causing depressed ribosomal RNA synthesis in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 183 ·No. 1 ·1981-00-00 ·Pages 54-8

Oostra BA, Kok K, Van Vliet AJ, Ab G, Gruber M

Abstract

Macromolecular synthesis in an Escherichia coli mutant with a temperature-sensitive beta' subunit of RNA polymerase was analysed. At the non-permissive temperature ribosomal RNA synthesis is strongly reduced while messenger RNA synthesis is affected to only a slightly extent. The overall protein synthesis is only slightly affected. We conclude that the beta' subunit is involved in promoter recognition and plays a role in transcriptional selectivity.

MeSH Terms
DNA-Directed RNA Polymerases/genetics Enzyme Precursors/genetics Escherichia coli/genetics Mutation Operon Phenotype RNA, Messenger/biosynthesis RNA, Ribosomal/biosynthesis Transcription, Genetic beta-Galactosidase/metabolism
Chemicals
Enzyme Precursors RNA, Messenger RNA, Ribosomal DNA-Directed RNA Polymerases beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Oostra B A
Kok K
Van Vliet A J
Ab G
Gruber M
References (24)
24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270138
Published
1981-00-00
Pages
54-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270138
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