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PMID: 6700588 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Species-specific rDNA transcription is due to promoter-specific binding factors.

Molecular and cellular biology ·Vol. 4 ·No. 2 ·1984-02-00 ·Pages 221-7

Miesfeld R, Arnheim N

Abstract

RNA polymerase I transcription factors were purified from HeLa and mouse L cell extracts by phosphocellulose chromatography. Three fractions from each species were found to be required for transcription. One of these fractions, virtually devoid of RNA polymerase I activity, was found to form a stable preinitiation complex with small DNA fragments containing promoter sequences from the homologous but not the heterologous species. These species-specific DNA-binding factors can explain nucleolar dominance in vivo in mouse-human hybrid somatic cells and species specificity in cell-free, RNA polymerase I-dependent transcription systems. The evolution of species-specific transcriptional control signals may be the natural outcome of a special relationship that exists between the RNA polymerase I transcription machinery and the multigene family coding for rRNA.

MeSH Terms
Animals DNA/metabolism DNA, Ribosomal HeLa Cells Humans Hybrid Cells/metabolism L Cells Mice Operon RNA Polymerase I/metabolism Species Specificity Templates, Genetic Transcription, Genetic
Chemicals
DNA, Ribosomal DNA RNA Polymerase I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miesfeld R
Arnheim N
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.2.221-227.1984
Published
1984-02-00
Pages
221-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368684
Subset
IM
Grants
NIGMS NIH HHS · GM25365 · United States
NIGMS NIH HHS · GM28632 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.2.221-227.1984 PMC Full Text View journal details
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