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PMID: 6592417 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Selective transduction of recombinant plasmids with cloned pac sites by Salmonella phage P22.

Molecular & general genetics : MGG ·Vol. 196 ·No. 1 ·1984-00-00 ·Pages 123-8

Schmidt C, Schmieger H

Abstract

Recombinant plasmids having PstI fragments of P22 DNA inserted in the vector pBR322 can be transduced efficiently by Salmonella phage P22, irrespective of the cloned phage sequences. When the rec function of the donor cells and the corresponding recombination system erf of the infecting phage are simultaneously inactivated, only plasmids containing the P22 pac site can be transduced. By this selective, generalized transduction an EcoRV DNA fragment of the P22 related phage L has been identified that carries a base sequence recognized by phage P22 as a packaging signal. Experiments in which only one of the two recombination systems was inactivated, showed that the bacterial rec system obviously promotes cointegrate formation between plasmid and phage DNA much more efficiently than the phage-coded erf system, allowing the specialized plasmid transduction observed by Orbach and Jackson (1982).

MeSH Terms
Cloning, Molecular Genes, Viral Plasmids Recombination, Genetic Salmonella/genetics Salmonella Phages/genetics Transduction, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schmidt C
Schmieger H
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334103
Published
1984-00-00
Pages
123-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334103
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