Home LiteratureArticle Details
PMID: 6757663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The packaging initiation site of phage P22. Analysis of packaging events by transduction.

Molecular & general genetics : MGG ·Vol. 187 ·No. 3 ·1982-00-00 ·Pages 510-5

Kufer B, Backhaus H, Schmieger H

Abstract

P22 lysates were grown on Salmonella strains carrying P22 prophages deleted to various extents. Transducing bacterial markers at both sides of the prophage insertion site it could be shown that: (i) transduction of markers can be enhanced by the prophage pac site; (ii) the recognition signal pac is in the area of gene 3 on the phage genome and thus close to the cutting site(s); (iii) transposon Tn10 may also act as a signal for packaging initiation; (iv) (at least) Tn10 initiates packaging sequences in both directions.

MeSH Terms
Genes, Viral Genotype Recombination, Genetic Salmonella Phages/genetics Salmonella typhimurium/genetics Species Specificity Transduction, Genetic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kufer B
Backhaus H
Schmieger H
References (21)
21 references, click to expand
  1. Replication in situ and DNA encapsulation following induction of an excision-defective lysogen of Salmonella bacteriophage P22.
    J Mol Biol. 1978 Jan 25;118(3):389-411 PMID: 344889
  2. Altered cotransduction frequencies exhibited by HT-mutants of Salmonella-phage P22.
    Mol Gen Genet. 1976 Feb 2;143(3):307-9 PMID: 1250221
  3. Non-random circular permutation of phage P22 DNA.
    J Mol Biol. 1974 Jan 5;85(4):501-28 PMID: 4853363
  4. Bacteriophage P22-mediated specialized transduction in Salmonella typhimurium: high frequency of aberrant prophage excision.
    J Virol. 1978 Sep;27(3):519-34 PMID: 359827
  5. Linkage map of Salmonella typhimurium, edition V.
    Microbiol Rev. 1978 Jun;42(2):471-519 PMID: 353483
  6. EcoRI analysis of bacteriophage P22 DNA packaging.
    J Mol Biol. 1978 Jan 25;118(3):365-88 PMID: 344888
  7. A phage P22 gene controlling integration of prophage.
    Virology. 1967 Feb;31(2):207-16 PMID: 6021093
  8. Effects of deletions on cotransduction linkage in Salmonella typhimurium: evidence that bacterial chromosome deletions affect the formation of transducing DNA fragments.
    Mol Gen Genet. 1974;131(2):97-112 PMID: 4607786
  9. Restriction endonuclease Hin dIII cleavage site map of bacteriophage P22.
    Virology. 1979 Jun;95(2):359-72 PMID: 380136
  10. Genetics of bacteriophage P22. I. Isolation of prophage deletions which affect immunity to superinfection.
    Virology. 1972 Jul;49(1):257-67 PMID: 4556925
  11. Altered linkage values in phage P22--mediated transduction caused by distant deletions or insertions in donor chromosomes.
    Mol Gen Genet. 1979 Oct 2;176(1):87-93 PMID: 232232
  12. A mutant of phage P22 with randomly permuted DNA.
    J Mol Biol. 1976 Jan 25;100(3):421-6 PMID: 1255717
  13. Phage genes involved in the formation generalized transducing particles in Salmonella--Phage P22.
    Mol Gen Genet. 1974;135(2):175-84 PMID: 4457756
  14. Evidence that HT mutant strains of bacteriophage P22 retain an altered form of substrate specificity in the formation of transducing particles in Salmonella typhimurium.
    Genet Res. 1976 Apr;27(2):315-22 PMID: 776744
  15. Initiation of sequential packaging of bacteriophage P22 DNA.
    J Mol Biol. 1982 May 15;157(2):287-98 PMID: 6286978
  16. [Molecular structure of transducing particles in the Salmonella phage P22. I. Density gradient studies on intact phages].
    Mol Gen Genet. 1968;102(4):336-47 PMID: 5743435
  17. Specialized transduction by bacteriophage P22 in Salmonella typhimurium: genetic and physical structure of the transducing genomes and the prophage attachment site.
    Genetics. 1976 Jul;83(3 PT.2):433-58 PMID: 783004
  18. Phage P22-mutants with increased or decreased transduction abilities.
    Mol Gen Genet. 1972;119(1):75-88 PMID: 4564719
  19. Chromosome Fragments Participating in Transduction in Salmonella Typhimurium.
    Genetics. 1959 May;44(3):457-70 PMID: 17247838
  20. Bacteriophage P22-mediated specialized transduction in Salmonella typhimurium: identification of different types of specialized transducing particles.
    J Virol. 1978 Sep;27(3):535-50 PMID: 359828
  21. Defective phage formation by lysogens of integration deficient phage P22 mutants.
    Virology. 1968 Feb;34(2):203-23 PMID: 4867906
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332636
Published
1982-00-00
Pages
510-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332636
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2