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PMID: 6752658 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mechanism of pBR322 transduction mediated by cytosine-substituting T4 bacteriophage.

Molecular & general genetics : MGG ·Vol. 186 ·No. 4 ·1982-00-00 ·Pages 497-500

Takahashi H, Saito H

Abstract

A cytosine-substitution type mutant of bacteriophage T4 (T4dC phage) has been shown to mediate the transfer of plasmid pBR322. The transduction frequency was around 10(-2) per singly infected cell at low multiplicity of infection. The transductants contained either a monomer or multimers of pBR322. The transducing capacity of T4dC phage was resistant to methylmethanesulfonate treatment. The results of Southern blotting experiments have indicated that the pBR322 DNA exists as head-to-tail concatemers in the transducing particles. The mechanism of transfer of pBR322 mediated by T4dC phages is discussed.

MeSH Terms
Cytosine Electrophoresis, Agar Gel Escherichia coli/genetics Mutation Phenotype Plasmids T-Phages/genetics Transduction, Genetic
Chemicals
Cytosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Takahashi H
Saito H
References (13)
13 references, click to expand
  1. In vitro construction of bacteriophage lambda and plasmid DNA molecules containing DNA fragments from bacteriophage T4.
    Gene. 1976;1(1):93-106 PMID: 802392
  2. A gene of bacteriophage T4 whose product prevents true late transcription on cytosine-containing T4 DNA.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3098-102 PMID: 1067605
  3. Studies of viable T4 bacteriophage containing cytosine-substituted DNA (T4dC phage). II. Cleavage of T4dC DNA by endonuclease SalI and bam HI.
    Mol Gen Genet. 1979 Jan 5;168(1):49-53 PMID: 372740
  4. Molecular cloning of fragments of bacteriophage T4 DNA.
    Mol Gen Genet. 1977 Nov 14;156(2):203-14 PMID: 600265
  5. The genome of bacteriophage T4.
    Bacteriol Rev. 1976 Dec;40(4):847-68 PMID: 795415
  6. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  7. Design of a system of conditional lethal mutations (tab/k/com) affecting protein-protein interactions in bacteriophage T4-infected Escherichia coli.
    J Mol Biol. 1975 Aug 25;96(4):563-78 PMID: 1104861
  8. High-frequency generalised transduction by bacteriophage T4.
    Nature. 1979 Jul 5;280(5717):80-2 PMID: 15305587
  9. The BAM H1 restriction site in the bacteriophage T4 chromosome is located in or near gene 8.
    Genetics. 1979 Oct;93(2):285-96 PMID: 231540
  10. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  11. A restriction map of the bacteriophage T4 genome.
    Mol Gen Genet. 1980;179(2):421-435 PMID: 6258018
  12. CHROMOSOME STRUCTURE IN PHAGE T4. I. CIRCULARITY OF THE LINKAGE MAP.
    Proc Natl Acad Sci U S A. 1964 May;51:775-9 PMID: 14172990
  13. Genetic and physiological characterization of Escherichia coli K12 mutants (tabC) which induce the abortive infection of bacteriophage T4.
    Virology. 1978 Jun 15;87(2):256-65 PMID: 351929
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337955
Published
1982-00-00
Pages
497-500
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337955
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