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PMID: 6576380 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proenkephalin, [Met]enkephalin, and oxytocin immunoreactivities are colocalized in bovine hypothalamic magnocellular neurons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 16 ·1983-08-00 ·Pages 5139-43

Vanderhaeghen JJ, Lotstra F, Liston DR, Rossier J

Abstract

The distribution of proenkephalin and [Met]enkephalin immunoreactivities in the bovine hypothalamo-neurohypophyseal system was studied by use of specific antisera. Proenkephalin and [Met]enkephalin immunoreactivities were found in magnocellular neuronal cell bodies in the dorsal part of the supraoptic nuclei and in the peripheral part of the paraventricular nuclei. A densely staining network of nerve terminals was found in the external part of the median eminence and in the posterior hypophysis. This general distribution is identical to that of the neurohypophyseal hormone oxytocin. The precise localization of proenkephalin and [Met]enkephalin immunoreactivities was compared to the distribution of oxytocin and vasopressin in serial 5-micron sections through the magnocellular nuclei. Oxytocin immunoreactivity was nearly always present in cells that were stained with proenkephalin and [Met]enkephalin antisera. The vasopressin-immunoreactive cells were never stained with either the proenkephalin or the [Met]enkephalin antisera.

MeSH Terms
Animals Cattle Enkephalin, Methionine/analysis Enkephalins/analysis Hypothalamo-Hypophyseal System/cytology Hypothalamus/cytology Immune Sera Immunoassay Neurons/analysis Organ Specificity Oxytocin/analysis Protein Precursors/analysis Supraoptic Nucleus/cytology
Chemicals
Enkephalins Immune Sera Protein Precursors proenkephalin Oxytocin Enkephalin, Methionine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vanderhaeghen J J
Lotstra F
Liston D R
Rossier J
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.16.5139
Published
1983-08-00
Pages
5139-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384205
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.16.5139 PMC Full Text View journal details
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