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PMID: 6265943 Published · ppublish English Journal Article

A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]- and [Leu]enkephalin sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 5 ·1981-05-00 ·Pages 3265-8

Kilpatrick DL, Taniguchi T, Jones BN, Stern AS, Shively JE, Hullihan J, Kimura S, Stein S, Udenfriend S

Abstract

A 3200-dalton adrenal enkephalin-containing peptide, designated peptide E, that exhibits high opiate activity in the guinea pig ileum longitudinal muscle preparation was purified, and its structure was determined. It contains an amino-terminal [Met]enkephalin sequence and a [Leu]enkephalin sequence at the carboxyl terminus. Sequence analysis revealed that peptide E arises from a previously characterized 4900-dalton adrenal enkephalin-containing peptide. Peptide E was shown to be 30 times more potent than [Met]enkephalin in the guinea pig ileum assay, which suggests that the adrenal enkephalin-containing peptide may perform a unique biological function in vivo.

MeSH Terms
Adrenal Medulla/analysis Amino Acid Sequence Animals Biological Assay Cattle Cell Line Chromaffin Granules/analysis Chromaffin System/analysis Endorphins/metabolism Enkephalins/classification,metabolism Glioma Guinea Pigs Ileum/drug effects Mice Molecular Weight Muscle Contraction/drug effects Neuroblastoma Receptors, Opioid/metabolism
Chemicals
Endorphins Enkephalins Receptors, Opioid adrenal opioid peptide E
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kilpatrick D L
Taniguchi T
Jones B N
Stern A S
Shively J E
Hullihan J
Kimura S
Stein S
Udenfriend S
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19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.5.3265
Published
1981-05-00
Pages
3265-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319542
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.5.3265 PMC Full Text View journal details
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