Home LiteratureArticle Details

PMID: 6571986 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cloning of the Vibrio harveyi luciferase genes: use of a synthetic oligonucleotide probe.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 1 ·1983-01-00 ·Pages 120-3

Cohn DH, Ogden RC, Abelson JN, Baldwin TO, Nealson KH, Simon MI, Mileham AJ

Abstract

A mixed-sequence synthetic oligonucleotide probe was used to isolate a clone containing the gene encoding the alpha subunit of bacterial luciferase from Vibrio harveyi and part of the gene coding for the beta subunit. DNA sequence analysis has allowed us to determine that the genes are closely linked on the bacterial chromosome and transcribed in the same direction. Comparison of the sequences in the regions preceding the two structural genes has revealed considerable homology and has identified sites that may be involved in the expression of the genes. Identification of a clone from a clone bank of total genomic DNA from this organism shows that mixed probes can be successfully used to isolate a gene of interest from any bacterium provided some protein sequence for the gene product is available.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular Genes Luciferases/genetics Macromolecular Substances Nucleic Acid Hybridization Oligodeoxyribonucleotides Vibrio/genetics

Chemicals

Macromolecular Substances Oligodeoxyribonucleotides Luciferases

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Cohn D H

Ogden R C

Abelson J N

Baldwin T O

Nealson K H

Simon M I

Mileham A J

References (23)

23 references, click to expand

Cellular control of the synthesis and activity of the bacterial luminescent system.
J Bacteriol. 1970 Oct;104(1):313-22 PMID: 5473898
Bacterial luciferase. The hydrophobic environment of the reactive sulfhydryl.
J Biol Chem. 1974 Apr 25;249(8):2393-6 PMID: 4822499
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
Lambdoid phages that simplify the recovery of in vitro recombinants.
Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
Autoinduction of bacterial luciferase. Occurrence, mechanism and significance.
Arch Microbiol. 1977 Feb 4;112(1):73-9 PMID: 843170
Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
Bacterial bioluminescence.
Annu Rev Microbiol. 1977;31:549-95 PMID: 199107
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
Chemical synthesis of versatile adaptors for molecular cloning.
Biochem Biophys Res Commun. 1978 Apr 14;81(3):695-703 PMID: 96825
Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.
Nucleic Acids Res. 1979 Aug 10;6(11):3543-57 PMID: 158748
Covalent structure of subunits of bacterial luciferase: NH2-terminal sequence demonstrates subunit homology.
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4887-9 PMID: 315557
Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.
Nucleic Acids Res. 1980 Nov 25;8(22):5473-89 PMID: 7465418
Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.
Nucleic Acids Res. 1980 Nov 25;8(22):5491-505 PMID: 7465419
Inactivation of luciferase from the Luminous marine bacterium Beneckea harveyi by proteases: evidence for a protease labile region and properties of the protein following inactivation.
Arch Biochem Biophys. 1980 Dec;205(2):554-63 PMID: 6970544
A system for shotgun DNA sequencing.
Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
Translational initiation in prokaryotes.
Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6613-7 PMID: 6171820
Partial characterization of the mRNA that codes for enkephalins in bovine adrenal medulla and human pheochromocytoma.
Proc Natl Acad Sci U S A. 1982 Jan;79(2):360-4 PMID: 6952189

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.80.1.120

Published

1983-01-00

Pages

120-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC393321

Subset

Databases

GENBANK

J01848, J01849, J01850

External Links

PubMed source DOI: 10.1073/pnas.80.1.120 PMC Full Text View journal details

Analysis Services

Cloning of the Vibrio harveyi luciferase genes: use of a synthetic oligonucleotide probe.

Contact

WeChat Official Account