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PMID: 6547776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A cytological map of the human X chromosome--evidence for non-random recombination.

Nucleic acids research ·Vol. 12 ·No. 13 ·1984-07-11 ·Pages 5277-85

Hartley DA, Davies KE, Drayna D, White RL, Williamson R

Abstract

The cytological location of six cloned DNA sequences on the human X chromosome has been determined to a high resolution by direct hybridisation 'in situ' to metaphase chromosomes. Each locus has been identified using clones which also detect restriction fragment length polymorphisms by Southern hybridisation. The six loci identified are spaced along the chromosome from Xp22 to Xq28. By combining data obtained using this powerful sequence localisation technique with that from hybrid cell panels and from family studies, it is possible to compare physical and genetic distances, and to demonstrate that the frequency of reciprocal genetic exchange is not uniform along the chromosome length.

MeSH Terms
Base Sequence Chromosome Banding Chromosome Mapping Cloning, Molecular DNA/analysis Female Humans Karyotyping Metaphase Nucleic Acid Hybridization X Chromosome
Chemicals
DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hartley D A
Davies K E
Drayna D
White R L
Williamson R
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.13.5277
Published
1984-07-11
Pages
5277-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318919
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.13.5277 PMC Full Text View journal details
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