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PMID: 6458043 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional cooperation of the dnaE and dnaN gene products in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 9 ·1981-09-00 ·Pages 5764-7

Kuwabara N, Uchida H

Abstract

A system was designed to isolate second-site intergenic suppressors of a thermosensitive mutation of the dnaE gene of Escherichia coli. The dnaE gene codes for the alpha subunit of DNA polymerase III [McHenry, C. S. & Crow, W. (1979) J. Biol. Chem. 254, 1748-1753]. One such suppressor, named sueA77, was finely mapped and found to be located at 82 min on the E. coli chromosome, between dnaA and recF, and within the dnaN gene [Sakakibara, Y. & Mizukami, T. (1980) Mol. Gen. Genet. 178, 541-553]. The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme [Burgers, P. M. J., Kornberg, A. & Sakakibara, Y. (1981) Proc. Natl. Acad. Sci. USA 78, 5391-5395]. The sueA77 mutation was trans-dominant over its wild-type allele, and it suppressed different thermosensitive mutations of dnaE with different maximal permissive temperature. These properties were interpreted as providing genetic evidence for interaction of the dnaE and dnaN gene products in E. coli.

MeSH Terms
Bacteriophage lambda DNA Polymerase III/genetics DNA Replication DNA-Directed DNA Polymerase/genetics Escherichia coli/genetics Genes, Bacterial Macromolecular Substances Suppression, Genetic Transduction, Genetic
Chemicals
Macromolecular Substances DNA Polymerase III DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kuwabara N
Uchida H
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.9.5764
Published
1981-09-00
Pages
5764-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348855
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.9.5764 PMC Full Text View journal details
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