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PMID: 395417 Published · ppublish English Journal Article

Integration of IS3 into IS2 generates a short sequence duplication.

Molecular & general genetics : MGG ·Vol. 177 ·No. 1 ·1979-00-00 ·Pages 85-9

Sommer H, Cullum J, Saedler H

Abstract

The Gal+ allele IS2-43 is known to segregate Gal- clones. Among 11 Gal- segregants, one was shown to be due to the integration of IS3 into IS2-43. Precise excision of the integrated IS3 element occurred at a rate of 5 x 10(-9)/cell/generation. DNA sequence analysis revealed that the termini of the IS3 element have the relation of imperfect inverted repeats and it is now flanked by a 3bp or 4bp duplication, a size which has not been seen before with other elements.

MeSH Terms
Alleles Base Sequence Chromosome Mapping Chromosomes, Bacterial DNA Transposable Elements DNA, Bacterial Escherichia coli/genetics
Chemicals
DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sommer H
Cullum J
Saedler H
References (26)
26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267256
Published
1979-00-00
Pages
85-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267256
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