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PMID: 6458017 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription of sea urchin histone genes in Escherichia coli.

Nucleic acids research ·Vol. 9 ·No. 16 ·1981-08-25 ·Pages 3889-906

Mellado RP, Delius H, Klein B, Murray K

Abstract

DNA fragments comprising units of the repeated histone genes form the sea urchins Psammechinus miliaris and Echinus esculentus were placed under the control of bacteriophage Lambda promoters by cloning into lambda replacement vectors. Although promoter-like regions exist within the cloned fragments, transcription of the histone genes is controlled mainly, but not exclusively, by lambda PL promoter. A transcription map of the cloned P. miliaris histone DNA fragment was obtained. The order of histone genes in E. esculentus was deduced from electron microscopic analyses of heteroduplexes with P. miliaris histone genes, and is similar to that in P. miliaris. Translation products of the transcripts have not been found in E. coli.

MeSH Terms
Animals Bacteriophage lambda/genetics Chromosome Mapping DNA, Recombinant/metabolism Escherichia coli/genetics Histones/genetics Plasmids Protein Biosynthesis RNA, Messenger/genetics Sea Urchins/genetics Transcription, Genetic
Chemicals
DNA, Recombinant Histones RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mellado R P
Delius H
Klein B
Murray K
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52 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.16.3889
Published
1981-08-25
Pages
3889-906
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327403
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.16.3889 PMC Full Text View journal details
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