Novel screening procedure for recombinant plasmids.

Science (New York, N.Y.) ·Vol. 195 ·No. 4276 ·1977-01-28 ·Pages 391-3

Telford J, Boseley P, Schaffner W, Birnstiel M

Abstract

Lysed bacterial colonies containing potential recombinant plasmids were mixed with molten agar and sealed into slots of an agarose gel. After electrophoresis overnight, the gel was stained with ethidium bromide, which clearly reveals recombinant plasmids. Xenopus laevis ribosomal DNA and histone DNA of Psammechinus miliaris were ligated into pCRI plasmids and screened by this method.

MeSH Terms

Animals DNA, Bacterial/analysis DNA, Recombinant Echinodermata Electrophoresis, Agar Gel Escherichia coli Extrachromosomal Inheritance Genes Genetic Engineering Histones Methods Plasmids RNA, Ribosomal Recombination, Genetic Xenopus

Chemicals

DNA, Bacterial DNA, Recombinant Histones RNA, Ribosomal

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Telford J

Boseley P

Schaffner W

Birnstiel M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.318763

Published

1977-01-28

Pages

391-3

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.318763 View journal details

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