Home LiteratureArticle Details
PMID: 6450313 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin.

Molecular & general genetics : MGG ·Vol. 179 ·No. 1 ·1980-00-00 ·Pages 49-54

Lengeler J

Abstract

From cultures of sensitive bacteria, treated with the antibiotic streptozotocin, two classes of resistant mutants can be isolated: 1) mutants, resistant under all the conditions tested to even the highest doses of the antibiotic. These are either pleiotropic-defective, pts-mutants, or more frequently, mutants lacking a transport system (enzyme IINag-complex of the PEP-dependent phosphotransferase system) encoded by the gene nagE. This gene is inducible by N-acetyl-glucosamine and seems to be part of the nag operon. The transport system in question is responsible for the uptake of N-acetyl-glucosamine, of D-glucosamine and of streptozotocin; 2) conditional resistant mutants which are unable to energize or to synthesize the streptozotocin transport system under certain growth conditions but do have the transport activity under other conditions. These include a) mutants auxotrophic for amino acids, vitamins, or nucleotides, b) mutants negative or sensitive to carbohydrates in the medium, and c) mutants wth defects in energy metabolism such as PEP synthesis.

MeSH Terms
Dose-Response Relationship, Drug Drug Resistance, Microbial Escherichia coli/genetics,isolation & purification Mutation Phenotype Streptozocin/pharmacology
Chemicals
Streptozocin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lengeler J
References (21)
21 references, click to expand
  1. The genetics of bacterial transport systems.
    Annu Rev Genet. 1970;4:225-62 PMID: 4950060
  2. The mechanism of the irreversible antimicrobial effects of penicillins: how the beta-lactam antibiotics kill and lyse bacteria.
    Annu Rev Microbiol. 1979;33:113-37 PMID: 40528
  3. Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping.
    J Bacteriol. 1975 Oct;124(1):26-38 PMID: 1100602
  4. Nalidixic acid for enrichment of auxotrophs in cultures of Salmonella typhimurium.
    Appl Microbiol. 1974 Oct;28(4):579-81 PMID: 4607841
  5. Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase.
    Nature. 1978 Oct 5;275(5679):420-3 PMID: 211433
  6. Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli.
    Mol Gen Genet. 1978 Apr 25;161(1):89-98 PMID: 353502
  7. Nalidixic acid-resistant auxotrophs of Escherichia coli.
    J Bacteriol. 1970 Nov;104(2):1027-9 PMID: 4923064
  8. Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase.
    J Bacteriol. 1979 Apr;138(1):40-7 PMID: 108253
  9. [Studies on the glucose effect in the synthesis of the galactose enzyme of Escherichia coli].
    Z Vererbungsl. 1966;98(3):203-29 PMID: 4863695
  10. Analysis of the physiological effects of the antibiotic streptozotocin on Escherichia coli K 12 and other sensitive bacteria.
    Arch Microbiol. 1980 Dec;128(2):196-203 PMID: 6452103
  11. Analysis of mutations affecting the dissmilation of galactitol (dulcitol) in Escherichia coli K 12.
    Mol Gen Genet. 1977 Mar 28;152(1):83-91 PMID: 325390
  12. Analysis of regulatory mechanisms controlling the activity of the hexitol transport systems in Escherichia coli K12.
    Mol Gen Genet. 1978 Nov 16;167(1):75-82 PMID: 368587
  13. The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-D-glucosamine by Escherichia coli.
    Biochem J. 1970 Jun;118(1):89-92 PMID: 4919472
  14. Use of dihydrostreptomycin for the isolation of auxotrophic mutants of Pseudomonas aeruginosa.
    J Bacteriol. 1966 Mar;91(3):1387 PMID: 4160232
  15. Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants.
    J Bacteriol. 1978 Jan;133(1):149-57 PMID: 201606
  16. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  17. Regulation of glycerol catabolism in Klebsiella aerogenes.
    J Bacteriol. 1974 Jul;119(1):50-6 PMID: 4366250
  18. Mutations affecting amino sugar metabolism in Escherichia coli K-12.
    J Bacteriol. 1972 Jul;111(1):290-1 PMID: 4591481
  19. The bacterial phosphoenolpyruvate: sugar phosphotransferase system.
    Biochim Biophys Acta. 1976 Dec 14;457(3-4):213-57 PMID: 187249
  20. [Membrane permeability and antibiotic resistance in bacteria].
    Naturwissenschaften. 1977 Mar;64(3):126-32 PMID: 321973
  21. Control of amino sugar metabolism in Escherichia coli and isolation of mutants unable to degrade amino sugars.
    Biochem J. 1968 Feb;106(4):847-58 PMID: 4866432
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268445
Published
1980-00-00
Pages
49-54
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268445
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2