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PMID: 6425021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A DNA sequence of Drosophila melanogaster with a differential telomeric distribution.

Chromosoma ·Vol. 89 ·No. 3 ·1984-00-00 ·Pages 206-11

Renkawitz-Pohl R, Bialojan S

Abstract

A DNA sequence (8-19T) of 2.3 kilobase pairs (kb) of Drosophila melanogaster was localized by in situ hybridization to the extreme ends of polytene chromosomes and to the chromocenter. The relative abundance of this sequence at the ends of polytene chromosomes X:2L:2R:3L:3R is 1:3.4:1.9:0:2.7. This differential distribution is probably due to different copy numbers at the individual telomeric regions. Restriction enzyme analysis of genomic DNA shows that 8-19T sequences are interspersed with other sequences. The clone 8-19T, which contains most of this interspersed repetitive sequence, is itself not internally repetitive but has a complex sequence composition. Some of these sequences are transcribed into poly(A)+RNA. We suggest that the ends of Drosophila chromosomes are of a complex arrangement with some sequences common to all ends.

MeSH Terms
Animals Base Sequence Chromosomes/ultrastructure Cloning, Molecular DNA/genetics Drosophila melanogaster/genetics Nucleic Acid Hybridization
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Renkawitz-Pohl R
Bialojan S
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24 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00295001
Published
1984-00-00
Pages
206-11
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00295001 View journal details
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