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PMID: 6255425 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of cloned ribosomal DNA from Drosophila hydei.

Nucleic acids research ·Vol. 8 ·No. 20 ·1980-10-24 ·Pages 4593-611

Renkawitz-Pohl R, Glätzer KH, Kunz W

Abstract

The structure of ribosomal genes from the fly Drosophila hydei has been analyzed. EcoRI fragments, cloned in a plasmid vector, were mapped by restriction enzyme analysis. The lengths of the regions coding for 18S and 28S rRNA were defined by R-loop formation. From these data a physical map of the rRNA genes was constructed. There are two major types of rDNA units in D. hydei, one having a size of 11 kb and the other a size of 17 kb. The 17 kb unit results from an intervening sequence (ivs) of 6.0 kb, interrupting the beta-28S rRNA coding region. Some homology between th D. hydei ivs and D. melanogaster type 1 ivs has been described previously (1). However, the restriction sites within these ivs show considerable divergence. Whereas D. hydei rDNA D. melanogaster rDNA, the nontranscribed spacer has little, if any, sequence homology. Despite difference in sequence, D. hydei and D. melanogaster spacers show structural similarities in that both contain repeated sequence elements of similar size and location.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Drosophila/metabolism Genes Microscopy, Electron Molecular Weight Nucleic Acid Conformation Plasmids RNA, Ribosomal/biosynthesis
Chemicals
DNA, Recombinant RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Renkawitz-Pohl R
Glätzer K H
Kunz W
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.20.4593
Published
1980-10-24
Pages
4593-611
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324373
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.20.4593 PMC Full Text View journal details
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