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PMID: 6417454 Published · ppublish English Comparative Study Journal Article

Plasmid transformation in Bacillus subtilis. Effects of the insertion of Bacillus subtilis rRNA genes into plasmids.

Molecular & general genetics : MGG ·Vol. 192 ·No. 1-2 ·1983-00-00 ·Pages 149-54

Iglesias A, Ceglowski P, Trautner TA

Abstract

Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in transformation of B. subtilis cells. The 2.3 Kb fragment could neither be subcloned in pC194 nor in pPL603, using both cell and protoplast transformation. We attribute the nonclonability of the 2.3 Kb fragment in B. subtilis to the presence of strong promoter activity in this fragment. Direct proof for the presence of a strong promoter, which is apparently responsible for the transcription of the rRNA gene, was obtained in experiments with B. subtilis and E. coli promoter search plasmids.

MeSH Terms
Bacillus subtilis/genetics Cloning, Molecular DNA/genetics DNA, Bacterial/genetics DNA, Ribosomal Escherichia coli/genetics Genes, Bacterial Operon Plasmids RNA, Ribosomal/genetics Transformation, Genetic
Chemicals
DNA, Bacterial DNA, Ribosomal RNA, Ribosomal DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Iglesias A
Ceglowski P
Trautner T A
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31 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327660
Published
1983-00-00
Pages
149-54
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327660
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