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PMID: 6406684 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro synthesis of late bacteriophage phi 29 RNA.

Journal of virology ·Vol. 46 ·No. 3 ·1983-06-00 ·Pages 690-702

Holder RD, Whiteley HR

Abstract

A crude P-100 fraction prepared from Bacillus subtilis 21 min after infection with wild-type phage phi 29 supported the in vitro synthesis of late phi 29 RNA by added RNA polymerase. Synthesis of late RNA was also detected when purified phi 29 DNA was transcribed by RNA polymerase in the presence of an S-150 fraction obtained by lysis of phi 29-infected cells in the presence of 1 M NaCl. Late phi 29 RNA was not synthesized when either the P-100 or the S-150 fraction was prepared from cultures infected with phi 29 having a mutation in gene 4.

MeSH Terms
Bacillus subtilis Bacteriophages/genetics,metabolism DNA-Directed RNA Polymerases/metabolism Genes, Viral RNA, Messenger/biosynthesis RNA, Viral/biosynthesis,genetics Sodium Chloride/pharmacology Temperature Transcription, Genetic
Chemicals
RNA, Messenger RNA, Viral Sodium Chloride DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Holder R D
Whiteley H R
References (41)
41 references, click to expand
  1. Transcription during the development of bacteriophage phi 29: production of host- and phi 29-specific ribonucleic acid.
    J Virol. 1972 Dec;10(6):1170-8 PMID: 4630153
  2. Synthesis in vitro of phi29-specific early proteins directed by phage DNA.
    Eur J Biochem. 1975 Feb 21;51(2):587-91 PMID: 807475
  3. Transcription in vitro of phi29 DNA and EcoRI fragments by Bacillus subtilis RNA polymerase.
    Eur J Biochem. 1976 Dec;71(1):77-83 PMID: 827446
  4. New phage-SPO1-induced polypeptides associated with Bacillus subtilis RNA polymerase.
    Proc Natl Acad Sci U S A. 1974 Jul;71(7):2761-5 PMID: 4212197
  5. An RNA-dependent nucleoside triphosphate phosphohydrolase (ATPase) associated with rho termination factor.
    Proc Natl Acad Sci U S A. 1974 May;71(5):2003-7 PMID: 4365581
  6. Termination factor for RNA synthesis.
    Nature. 1969 Dec 20;224(5225):1168-74 PMID: 4902144
  7. Electrophoretic strand separation of long DNAs with poly (U,G) in agarose gels.
    Nucleic Acids Res. 1978 Aug;5(8):2743-54 PMID: 99728
  8. Nucleotide sequences of transcription and translation initiation regions in Bacillus phage phi 29 early genes.
    J Biol Chem. 1982 Jan 25;257(2):1053-62 PMID: 6274853
  9. Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1978 Feb 25;119(2):269-91 PMID: 416224
  10. Effect of elevated temperature on deoxyribonucleic acid synthesis in bacteriophage phi-29-infected Bacillus amyloliquefaciens.
    J Virol. 1970 Oct;6(4):430-7 PMID: 5497888
  11. Complementary Strands of Bacteriophage phi29 Deoxyribonucleic Acid: Preparative Separation and Transcription Studies.
    J Virol. 1970 Dec;6(6):855-64 PMID: 16789128
  12. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. I. An assay for the rate and extent of ribonucleic acid chain initiation.
    J Biol Chem. 1974 May 25;249(10):2995-3001 PMID: 4598116
  13. Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections.
    J Virol. 1976 Aug;19(2):495-500 PMID: 822174
  14. In vitro system for middle T4 RNA. I. Studies with Escherichia coli RNA polymerase.
    J Biol Chem. 1982 Apr 25;257(8):4087-96 PMID: 7040377
  15. The interaction of Escherichia coli core RNA polymerase with specificity-determining subunits derived from unmodified and SP82-modified Bacillus subtilis RNA polymerase.
    J Biol Chem. 1980 Dec 25;255(24):11957-64 PMID: 6160156
  16. Gene expression during the development of Bacillus subtilis bacteriophage phi 29. I. Analysis of viral-specific transcription by deoxyribonucleic acid-ribonucleic acid competition hybridization.
    J Virol. 1973 Jan;11(1):78-86 PMID: 4630802
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  18. In vivo and in vitro transcription by ribonucleic acid polymerase from SP82-infected Bacillus subtilis.
    J Biol Chem. 1974 Mar 10;249(5):1483-9 PMID: 4206352
  19. Regulation of the expression of the N gene of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1973 Feb;70(2):421-4 PMID: 4568728
  20. Analysis of bacteriophage phi 29 gene function: protein synthesis in suppressor-sensitive mutant infection of Bacillus subtilis.
    J Virol. 1974 Jan;13(1):211-21 PMID: 4204249
  21. Transcription of the genome of bacteriophage phi 29: isolation and mapping of the major early mRNA synthesized in vivo and in vitro.
    J Virol. 1977 Sep;23(3):562-77 PMID: 408515
  22. Structure of Bacillus subtilis bacteriophage phi 29 and the length of phi 29 deoxyribonucleic acid.
    J Bacteriol. 1966 May;91(5):2081-9 PMID: 4957028
  23. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
    Virology. 1970 Mar;40(3):734-44 PMID: 4908735
  24. In vitro system for middle T4 RNA. II. Studies with T4-modified RNA polymerase.
    J Biol Chem. 1982 Apr 25;257(8):4097-101 PMID: 7040378
  25. Nuclear fraction of Bacillus subtilis as a template for ribonucleic acid synthesis.
    J Bacteriol. 1968 Apr;95(4):1221-37 PMID: 4296512
  26. Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102-6 PMID: 177975
  27. Transcription-termination factor Rho from Bacills subtilis.
    Eur J Biochem. 1980 Feb;104(1):313-20 PMID: 6445263
  28. Specificity of promoter site utilization in vitro by bacterial RNA polymerases on Bacillus phage phi 29 DNA. Transcription mapping with exonuclease III.
    J Biol Chem. 1980 Sep 25;255(18):8819-30 PMID: 6251067
  29. Identification of the protein firmly bound to the ends of bacteriophage phi 29 DNA.
    Virology. 1978 Feb;84(2):279-92 PMID: 203093
  30. Nucleotide sequence of the early genes 3 and 4 of bacteriophage phi 29.
    Nucleic Acids Res. 1982 Oct 11;10(19):5785-98 PMID: 6292852
  31. Transcription during the development of bacteriophage phi29: definition of "early" and "late" phi29 ribonucleic acid.
    J Virol. 1973 Jan;11(1):9-16 PMID: 4630804
  32. Nucleotide sequence of the major early region of bacteriophage phi 29.
    Gene. 1982 Mar;17(3):323-35 PMID: 6809534
  33. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  34. RNA polymerase from Bacillus amyloliquefaciens infected with phi29 bacteriophage.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2234-7 PMID: 4525426
  35. The role of the delta peptide of the Bacillus subtilis RNA polymerase in promoter selection.
    J Biol Chem. 1981 Jul 25;256(14):7424-32 PMID: 6788769
  36. High level synthesis in Escherichia coli of the Bacillus subtilis phage phi 29 proteins p3 and p4 under the control of phage lambda PL promoter.
    Nucleic Acids Res. 1982 Oct 11;10(19):5773-84 PMID: 6292851
  37. Bacteriophage SP82 induced modifications of Bacillus subtilis RNA polymerase result in the recognition of additional RNA synthesis initiation sites on phage DNA.
    Biochem Biophys Res Commun. 1978 Apr 14;81(3):1058-65 PMID: 96824
  38. Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: mapping and functional analysis of the head fiber gene.
    J Virol. 1977 Oct;24(1):363-77 PMID: 409854
  39. RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1979 Feb 5;127(4):411-36 PMID: 107317
  40. DNA strand specificity of temporal RNA classes produced during infection of Bacillus subtilis by SP82.
    J Virol. 1976 Aug;19(2):359-73 PMID: 822173
  41. Synthesis of virus-specific ribonucleic acid in KB cells infected with type 2 adenovirus.
    J Virol. 1971 Aug;8(2):203-14 PMID: 4256015
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.46.3.690-702.1983
Published
1983-06-00
Pages
690-702
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256545
Subset
IM
Grants
NIGMS NIH HHS · GM-20784 · United States
NIGMS NIH HHS · GM-26100 · United States
NIGMS NIH HHS · K6-GM-442 · United States
External Links
PubMed source DOI: 10.1128/JVI.46.3.690-702.1983 PMC Full Text View journal details
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