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PMID: 4568728 Published · ppublish English Journal Article

Regulation of the expression of the N gene of bacteriophage lambda.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 2 ·1973-02-00 ·Pages 421-4

Greenblatt J

Abstract

A quantitative assay for the N protein of bacteriophage lambda has been used to study the in vivo regulation of N gene expression. The assay makes use of the observation that in a cell-free protein-synthesizing system from Escherichia coli programmed with lambdaN(-) DNA the lambda endolysin is made only if N protein is added to the reaction. The rate of synthesis of N protein in vivo is negatively controlled by the products of the CI and tof genes of the phage. Furthermore, N protein activity is extremely unstable in vivo. During normal cell growth at 35 degrees , the half-life of N protein is about 2 min.

MeSH Terms
Cell-Free System Chromosome Mapping Coliphages/enzymology,metabolism Endopeptidases/analysis,biosynthesis Escherichia coli Genes Genes, Regulator Kinetics Lysogeny Temperature Transcription, Genetic Viral Proteins/analysis,biosynthesis,metabolism
Chemicals
Viral Proteins Endopeptidases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Greenblatt J
References (25)
25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.2.421
Published
1973-02-00
Pages
421-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433273
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.2.421 PMC Full Text View journal details
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