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PMID: 4525426 Published · ppublish English Journal Article

RNA polymerase from Bacillus amyloliquefaciens infected with phi29 bacteriophage.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 8 ·1973-08-00 ·Pages 2234-7

Holland M, Whiteley HR

Abstract

DNA-dependent RNA polymerase was purified from uninfected and varphi29-infected Bacillus amyloliquefaciens. Differences were observed in the specific activities, template specificities, stability, and sedimentation properties of the two enzymes. A polypeptide of 30,000 molecular weight was found in association with the polymerase of high specific activity from phage-infected cells and was absent from polymerase isolated from uninfected cells. The change in polymerase properties and the appearance of the polypeptide occurred early in phage infection.

MeSH Terms
Bacillus/enzymology Bacteriophages Centrifugation, Density Gradient Chromatography, Ion Exchange DNA, Bacterial DNA-Directed RNA Polymerases/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Lysogeny Molecular Weight Templates, Genetic
Chemicals
DNA, Bacterial DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Holland M
Whiteley H R
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.8.2234
Published
1973-08-00
Pages
2234-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433708
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.8.2234 PMC Full Text View journal details
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