An essential gene for replication of the mini-F plasmid from origin I.

Molecular & general genetics : MGG ·Vol. 196 ·No. 1 ·1984-00-00 ·Pages 59-63

Tanimoto K, Iino T

Abstract

We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Using these mutant mini-F plasmids, we found that F3 protein is essential for the replication of mini-F from origin I, but not from origin II.

MeSH Terms

Bacterial Proteins/genetics DNA Replication DNA, Recombinant Escherichia coli/genetics Gene Expression Regulation Genes Genes, Regulator Plasmids

Chemicals

Bacterial Proteins DNA, Recombinant

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tanimoto K

Iino T

References (20)

20 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00334092

Published

1984-00-00

Pages

59-63

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00334092

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An essential gene for replication of the mini-F plasmid from origin I.

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