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PMID: 6343840 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Comparative analysis of the 5'-end regions of two repressible acid phosphatase genes in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 3 ·No. 4 ·1983-04-00 ·Pages 570-9

Thill GP, Kramer RA, Turner KJ, Bostian KA

Abstract

The nucleotide sequence of 5'-noncoding and N-terminal coding regions of two coordinately regulated, repressible acid phosphatase genes from Saccharomyces cerevisiae were determined. These unlinked genes encode different, but structurally related polypeptides of molecular weights 60,000 and 56,000. The DNA sequences of their 5'-flanking regions show stretches of extensive homology upstream of, and surrounding, a "TATA" sequence and in a region in which heterogeneous 5' ends of the p60 mRNA were mapped. The predicted amino acid sequences encoded by the N-terminal regions of both genes were confirmed by determination of the amino acid sequence of the native exocellular acid phosphatase and the partial sequence of the presecretory polypeptide synthesized in a cell-free protein synthesizing system. The N-terminal region of the p60 polypeptide was shown to be characterized by a hydrophobic 17-amino acid signal polypeptide which is absent in the native exocellular protein and thought to be necessary for acid phosphatase secretion.

MeSH Terms
Acid Phosphatase/genetics Amino Acid Sequence Base Sequence DNA, Fungal/genetics Gene Expression Regulation Genes Genes, Regulator Peptides/physiology Protein Sorting Signals RNA, Messenger/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal Peptides Protein Sorting Signals RNA, Messenger Acid Phosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thill G P
Kramer R A
Turner K J
Bostian K A
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34 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.4.570-579.1983
Published
1983-04-00
Pages
570-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368572
Subset
IM
Grants
NCRR NIH HHS · S07 RR07044 · United States
Databases
GENBANK
J01344, J01345
External Links
PubMed source DOI: 10.1128/mcb.3.4.570-579.1983 PMC Full Text View journal details
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