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PMID: 6329733 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of mouse major urinary protein genes: different splicing configurations in the 3'-non-coding region.

The EMBO journal ·Vol. 3 ·No. 5 ·1984-05-00 ·Pages 1045-52

Clark AJ, Clissold PM, Al Shawi R, Beattie P, Bishop J

Abstract

The multigene family which codes for the mouse major urinary proteins (MUPs) consists of approximately 35 genes. Most of these are members of two different groups, Group 1 and Group 2, which can be distinguished by nucleic acid hybridisation. Here we describe the structure of a Group 1 gene and show that two size classes of MUP mRNA which are found in mouse liver result from different splicing events in the 3'-non-coding region and contain different polyadenylation sites. Short mRNA is approximately 750 nucleotides long, contains six exons, and is the main product of the Group 2 genes. Long mRNA is approximately 880 nucleotides long, contains seven exons and is the main product of the Group 1 genes. Five exons and part of the sixth are common to long and short mRNA and contain the coding region. This codes for an acidic protein of 180 amino acids containing an 18 residue signal peptide. A comparison of the mouse sequence with a homologous rat alpha 2u-globulin sequence shows that the rate of evolutionary divergence of the two proteins has been high. Silent sites have diverged four times more rapidly than replacement sites, showing that there has been selection against change in the protein sequence.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes Endonucleases Genes Mice Molecular Weight Plasmids Proteins/genetics RNA, Messenger/genetics Single-Strand Specific DNA and RNA Endonucleases
Chemicals
Proteins RNA, Messenger major urinary proteins Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Clark A J
Clissold P M
Al Shawi R
Beattie P
Bishop J
References (53)
53 references, click to expand
  1. Components of the major urinary protein complex of inbred mice: determination of NH2-terminal sequences and comparison with homologous components from wild mice.
    Biochem Genet. 1974 Apr;11(4):325-35 PMID: 4838000
  2. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  3. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  4. A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids.
    Cell. 1977 Aug;11(4):819-36 PMID: 890740
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. Analysis of mRNA populations by cDNA.mRNA hybrid-mediated inhibition of cell-free protein synthesis.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1217-21 PMID: 274713
  7. Regulation of mouse major urinary protein production by the Mup-A gene.
    Genetics. 1978 Nov;90(3):597-612 PMID: 730052
  8. Quantitative analysis of specific labelled RNA'S using DNA covalently linked to diazobenzyloxymethyl-paper.
    Nucleic Acids Res. 1979 Jan;6(1):195-203 PMID: 218170
  9. Multiple genes coding for the androgen-regulated major urinary proteins of the mouse.
    Cell. 1979 Jun;17(2):449-57 PMID: 88267
  10. The structure and transcription of four linked rabbit beta-like globin genes.
    Cell. 1979 Dec;18(4):1285-97 PMID: 519769
  11. In vitro synthesis and characterization of precursors to the mouse major urinary proteins.
    J Biol Chem. 1980 Feb 25;255(4):1367-73 PMID: 7354032
  12. Elution of DNA from agarose gels after electrophoresis.
    Methods Enzymol. 1979;68:176-82 PMID: 232211
  13. Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.
    Cell. 1980 Jun;20(2):313-9 PMID: 6771020
  14. The evolution of genes: the chicken preproinsulin gene.
    Cell. 1980 Jun;20(2):555-66 PMID: 7388949
  15. The ovalbumin gene family: structure of the X gene and evolution of duplicated split genes.
    Cell. 1980 Jul;20(3):625-37 PMID: 7418002
  16. Structure of a family of rat amylase genes.
    Nature. 1980 Sep 11;287(5778):117-22 PMID: 6159531
  17. Cloning and sequence analysis of cDNAs encoding two distinct somatostatin precursors found in the endocrine pancreas of anglerfish.
    Nature. 1980 Nov 13;288(5787):137-41 PMID: 6107860
  18. A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2.
    Nucleic Acids Res. 1980 Apr 25;8(8):1731-43 PMID: 6253946
  19. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.
    Cell. 1980 Oct;21(3):627-38 PMID: 7438203
  20. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
  21. Plasmid cloning vectors that can be nicked at a unique site.
    Mol Gen Genet. 1980;179(3):573-80 PMID: 6255286
  22. Exons encode functional and structural units of chicken lysozyme.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5759-63 PMID: 6934509
  23. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):5949-64 PMID: 6258156
  24. The primary transcription unit of the mouse beta-major globin gene.
    Cell. 1981 Feb;23(2):585-93 PMID: 6937266
  25. Amino acid sequence of the precursor of rat liver alpha 2 micro-globulin.
    J Biol Chem. 1981 Apr 25;256(8):3634-6 PMID: 6163771
  26. The role of DNA rearrangement and alternative RNA processing in the expression of immunoglobulin delta genes.
    Cell. 1981 May;24(2):353-65 PMID: 6786756
  27. Multiple polyadenylation sites in a mouse alpha-amylase gene.
    Nucleic Acids Res. 1981 May 25;9(10):2313-23 PMID: 6166922
  28. Cloning and sequence of several alpha 2u-globulin cDNAs.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3478-82 PMID: 6167987
  29. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  30. Human growth hormone DNA sequence and mRNA structure: possible alternative splicing.
    Nucleic Acids Res. 1981 Aug 11;9(15):3719-30 PMID: 6269091
  31. Molecular cloning of cDNA sequences transcribed from mouse liver endoplasmic reticulum poly(A)mRNA.
    Gene. 1981 Nov;15(2-3):225-35 PMID: 6117504
  32. Isolation of a cDNA clone for mouse urinary proteins: age- and sex-related expression of mouse urinary protein genes is transcriptionally controlled.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5425-9 PMID: 6946481
  33. A history of the human fetal globin gene duplication.
    Cell. 1981 Oct;26(2 Pt 2):191-203 PMID: 7332928
  34. Immunoglobulin heavy-chain expression and class switching in a murine leukaemia cell line.
    Nature. 1982 Mar 25;296(5855):325-31 PMID: 6801527
  35. Allelic variation at several different genetic loci determines the major urinary protein phenotype of inbred mouse strains.
    Genet Res. 1982 Feb;39(1):31-9 PMID: 7067971
  36. Nucleotide sequence surrounding multiple polyadenylation sites in the mouse dihydrofolate reductase gene.
    J Biol Chem. 1982 May 10;257(9):5143-7 PMID: 6121807
  37. Mapping the structural genes coding for the major urinary proteins in the mouse: combined use of recombinant inbred strains and somatic cell hybrids.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1220-4 PMID: 6951169
  38. Calcitonin mRNA polymorphism: peptide switching associated with alternative RNA splicing events.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1717-21 PMID: 6952224
  39. Pretranslational regulation of alpha 2u-globulin in rat liver by growth hormone.
    J Biol Chem. 1982 Jul 10;257(13):7834-8 PMID: 6177693
  40. Structural genes of the mouse major urinary protein are on chromosome 4.
    J Cell Biol. 1982 Aug;94(2):414-7 PMID: 7107707
  41. Variation in mouse major urinary protein (MUP) genes and the MUP gene products within and between inbred lines.
    Gene. 1982 Jun;18(3):211-20 PMID: 6290324
  42. Variation between mouse major urinary protein genes isolated from a single inbred line.
    Gene. 1982 Jun;18(3):221-30 PMID: 6290325
  43. The structure and expression of very closely related members of the alpha 2u globulin gene family.
    J Biol Chem. 1982 Nov 25;257(22):13527-34 PMID: 6183262
  44. A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing.
    Cell. 1982 Jul;29(3):903-11 PMID: 7151176
  45. Organization of the rat gamma-fibrinogen gene: alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen.
    Cell. 1982 Nov;31(1):159-66 PMID: 6897622
  46. Tissue-specific control of alpha 2u globulin gene expression: constitutive synthesis in the submaxillary gland.
    Cell. 1983 Feb;32(2):453-60 PMID: 6186396
  47. Alternative RNA splicing of the murine alpha A-crystallin gene: protein-coding information within an intron.
    Cell. 1983 Mar;32(3):707-12 PMID: 6187470
  48. The gene family for major urinary proteins: expression in several secretory tissues of the mouse.
    Cell. 1983 Mar;32(3):755-61 PMID: 6831559
  49. Specific transcription and RNA splicing defects in five cloned beta-thalassaemia genes.
    Nature. 1983 Apr 14;302(5909):591-6 PMID: 6188062
  50. Different forms of alpha 2u-globulin in male and female rat urine.
    Eur J Biochem. 1983 Jul 15;134(1):175-81 PMID: 6190651
  51. The pathway of eukaryotic mRNA formation.
    Annu Rev Biochem. 1983;52:441-66 PMID: 6193753
  52. Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.
    Nature. 1983 Oct 13-19;305(5935):600-5 PMID: 6194440
  53. Two main groups of mouse major urinary protein genes, both largely located on chromosome 4.
    EMBO J. 1982;1(5):615-20 PMID: 6329695
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-05-00
Pages
1045-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557469
Subset
IM
Databases
GENBANK
X03208
External Links
PubMed source PMC Full Text View journal details
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