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PMID: 6328490 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transforming potential of a human protooncogene (c-fps/fes) locus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 10 ·1984-05-00 ·Pages 3039-43

Sodroski JG, Goh WC, Haseltine WA

Abstract

The protooncogene c-fps/fes is a single vertebrate locus homologous to both the fps transforming gene of Fujinami avian sarcoma virus and the fes transforming gene of two feline sarcoma virus isolates. The human c-fps/fes locus has been previously cloned and characterized. We report that a recombinant gene in which 3' human c-fps/fes sequences replace over 80% of the feline sarcoma virus fes sequences transforms NIH 3T3 mouse fibroblasts and encodes a protein kinase. Cells transformed by this recombinant possess increased phosphotyrosine levels. These observations demonstrate that a large carboxyl portion of a human c-fps/fes protein product can functionally complement a retroviral transforming protein.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Base Sequence Cell Transformation, Neoplastic Cells, Cultured Cloning, Molecular DNA Restriction Enzymes Genes, Viral Humans Mice Mice, Inbred Strains Oncogenes Protein Kinases/metabolism Protein-Tyrosine Kinases Retroviridae/genetics Species Specificity Transfection
Chemicals
Protein Kinases Protein-Tyrosine Kinases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sodroski J G
Goh W C
Haseltine W A
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.10.3039
Published
1984-05-00
Pages
3039-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345216
Subset
IM
Grants
NCI NIH HHS · CA21082-04 · United States
NCI NIH HHS · CA26716-04 · United States
NCI NIH HHS · T32 CA09361-04 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.10.3039 PMC Full Text View journal details
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