Nucleotide sequence of cucumber pale fruit viroid: homology to hop stunt viroid.

Nucleic acids research ·Vol. 12 ·No. 8 ·1984-04-25 ·Pages 3427-34

Sano T, Uyeda I, Shikata E, Ohno T, Okada Y

Abstract

Double stranded cDNA of cucumber pale fruit viroid ( CPFV ) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170 (1982] and the complete nucleotide sequence was established. The covalently closed circular molecules of single-stranded CPFV RNA consists of 303 nucleotides. The nucleotide sequence of CPFV was compared with the previously established sequence of hop stunt viroid (HSV), which consists of 297 nucleotides ( Ohno et al. Nucleic Acid Res.11,6185-6197 (1983]. CPFV differs from HSV in the nucleotide sequence at 16 positions which include 8 exchanges, 7 insertions and 1 deletion. Both viroids share about 95% sequence homology. Considering the pathogenic properties of both viroids together, it is concluded that CPFV is a cucumber isolate of HSV.

MeSH Terms

Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes DNA, Viral/genetics Nucleic Acid Hybridization Plant Viruses/genetics Plasmids Species Specificity Viroids/genetics

Chemicals

DNA, Viral DNA DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Sano T

Uyeda I

Shikata E

Ohno T

Okada Y

References (14)

14 references, click to expand

Avocado sunblotch viroid: primary sequence and proposed secondary structure.
Nucleic Acids Res. 1981 Dec 11;9(23):6527-37 PMID: 7322921
Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid.
Eur J Biochem. 1982 Jan;121(2):249-57 PMID: 7060550
High-efficiency cloning of full-length cDNA.
Mol Cell Biol. 1982 Feb;2(2):161-70 PMID: 6287227
Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy.
Nucleic Acids Res. 1983 Sep 24;11(18):6185-97 PMID: 6312412
Structural similarities between viroids and transposable genetic elements.
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6234-8 PMID: 6312450
Citrus exocortis viroid: nucleotide sequence and secondary structure of an Australian isolate.
FEBS Lett. 1982 Jan 25;137(2):288-92 PMID: 15768484
Chrysanthemum stunt viroid: primary sequence and secondary structure.
Nucleic Acids Res. 1981 Jun 25;9(12):2741-52 PMID: 7279660
Nucleotide sequence of the rightward operator of phage lambda.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
Nucleotide sequence and secondary structure of potato spindle tuber viroid.
Nature. 1978 May 18;273(5659):203-8 PMID: 643081
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
Eur J Biochem. 1973 Aug 1;37(1):31-40 PMID: 4580885

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.8.3427

Published

1984-04-25

Pages

3427-34

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318759

Subset

Databases

GENBANK

X00524

External Links

PubMed source DOI: 10.1093/nar/12.8.3427 PMC Full Text View journal details

Analysis Services

Nucleotide sequence of cucumber pale fruit viroid: homology to hop stunt viroid.

Contact

WeChat Official Account