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PMID: 6312412 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy.

Nucleic acids research ·Vol. 11 ·No. 18 ·1983-09-24 ·Pages 6185-97

Ohno T, Takamatsu N, Meshi T, Okada Y

Abstract

The complete cDNA of hop stunt viroid (HSV) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170. (1982] and the complete nucleotide sequence has been established. The covalently closed circular single-stranded HSV RNA consists of 297 nucleotides. The secondary structure predicted for HSV contains 67% of its residues base-paired. The native HSV can possess an extended rod-like structure characteristic of viroids previously established. The central region of the native HSV has a similar structure to the conserved region found in all viroids sequenced so far except for avocado sunblotch viroid. The sequence homologous to the 5'-end of U1a RNA is also found in the sequence of HSV but not in the central conserved region.

MeSH Terms

Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes Genes, Viral Nucleic Acid Conformation Nucleic Acid Hybridization Plant Viruses/genetics RNA, Viral/genetics Viroids/genetics

Chemicals

RNA, Viral DNA DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ohno T

Takamatsu N

Meshi T

Okada Y

References (26)

26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.18.6185

Published

1983-09-24

Pages

6185-97

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326366

Subset

Databases

GENBANK

X00009

External Links

PubMed source DOI: 10.1093/nar/11.18.6185 PMC Full Text View journal details

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Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy.

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