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PMID: 16593138 Published · ppublish English Journal Article

RNA intermediates in potato spindle tuber viroid replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 1 ·1982-01-00 ·Pages 113-7

Owens RA, Diener TO

Abstract

Two double-stranded RNA intermediates of viroid replication have been isolated from potato spindle tuber viroid (PSTV)-infected tomato tissue and characterized by polyacrylamide gel electrophoresis and DNA-RNA hybridization techniques. These replicative intermediates contain monomeric circular or linear PSTV strands complexed with a multimeric complementary RNA strand. Synchronous synthesis of single-stranded PSTV is accompained by a simultaneous marked increase in double-stranded PSTV RNA; thus, in vivo precursors of the characterized double-stranded PSTV RNAs seem to be involved in PSTV replication. A "rolling circle" model for viroid replication on a circular PSTV template can accommodate the double-stranded PSTV RNA species characterized.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Owens R A
Plant Virology Laboratory, Plant Protection Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Diener T O
References (20)
20 references, click to expand
  1. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
    Methods Enzymol. 1979;68:220-42 PMID: 94421
  2. Molecular cloning and characterization of potato spindle tuber viroid cDNA sequences.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5302-6 PMID: 16592877
  3. Viroids: structure and function.
    Science. 1979 Aug 31;205(4409):859-66 PMID: 472709
  4. Common structural features of different viroids: serial arrangement of double helical sections and internal loops.
    Nucleic Acids Res. 1978 May;5(5):1589-610 PMID: 662695
  5. Double-stranded nucleic acids found in tissue infected with the satellite of tobacco ringspot virus.
    Virology. 1977 May 15;78(2):453-62 PMID: 867813
  6. Molecular weights of the double-stranded RNAs of cucumber mosaic virus strain S and its associated RNA 5.
    Virology. 1977 Jul 1;80(1):214-7 PMID: 878314
  7. Homogenization-resistant and -susceptible components of tobacco mosaic virus replicative form RNA.
    J Gen Virol. 1976 Aug;32(2):205-15 PMID: 1018172
  8. Differential temperature treatment of plants greatly enhances multiplication rates.
    Virology. 1973 Jun;53(2):476-8 PMID: 4712392
  9. Assay for secondary structure in ribonucleic acid.
    J Virol. 1972 Jun;9(6):903-8 PMID: 5064438
  10. Purification and properties of the replicative intermediate of the RNA bacteriophage R17.
    Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504-11 PMID: 5227669
  11. A new model for virus ribonucleic acid replication.
    Nature. 1965 Nov 27;208(5013):861-3 PMID: 5870101
  12. Viroids: a class of subviral pathogens.
    Angew Chem Int Ed Engl. 1980;19(4):231-43 PMID: 6155812
  13. Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
    Nucleic Acids Res. 1980 Jan 25;8(2):299-317 PMID: 6158735
  14. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  15. Nuclear DNA from uninfected or potato spindle tuber viroid-infected tomato plants contains no detectable sequences complementary to cloned double-stranded viroid cDNA.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6932-5 PMID: 6273895
  16. DNA-dependent RNA polymerase II of plant origin transcribes viroid RNA into full-length copies.
    Nature. 1981 May 28;291(5813):297-301 PMID: 7231549
  17. Detection of complementary RNA intermediates of viroid replication by Northern blot hybridization.
    Biosci Rep. 1981 Apr;1(4):327-36 PMID: 7295896
  18. Hybridization of potato spindle tuber viroid to cellular DNA of normal plants.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2453-7 PMID: 16592335
  19. RNA sequences complementary to citrus exocortis viroid in nucleic acid preparations from infected Gynura aurantiaca.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):896-900 PMID: 16592500
  20. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.1.113
Published
1982-01-00
Pages
113-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345672
External Links
PubMed source DOI: 10.1073/pnas.79.1.113 PMC Full Text View journal details
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