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PMID: 6327723 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphoinositide metabolism and the morphology of human erythrocytes.

The Journal of cell biology ·Vol. 98 ·No. 6 ·1984-06-00 ·Pages 1992-8

Ferrell JE, Huestis WH

Abstract

ATP-depleted human erythrocytes lose their smooth discoid shape and adopt a spiny, crenated form. This shape change coincides with the conversion of phosphatidylinositol-4,5-bisphosphate to phosphatidylinositol and phosphatidic acid to diacylglycerol. Both crenation and lipid dephosphorylation are accelerated by iodoacetamide, and both are reversed by nutrient supplementation. The observed changes in lipid populations should shrink the membrane inner monolayer by 0.6%, consistent with estimates of bilayer imbalance in crenated cells. These observations suggest that metabolic crenation arises from a loss of inner monolayer area secondary to the degradation of phosphatidylinositol-4,5-bisphosphate and phosphatidic acid. A related process, crenation after Ca2+ loading, appears to arise from a loss inositides by a different pathway.

MeSH Terms
Adenosine Triphosphate/blood Erythrocyte Membrane/ultrastructure Erythrocytes/drug effects,physiology,ultrastructure Humans Iodoacetamide/pharmacology Kinetics Microscopy, Electron, Scanning Phosphatidylinositols/blood Phospholipids/blood Phosphorus Radioisotopes
Chemicals
Phosphatidylinositols Phospholipids Phosphorus Radioisotopes Adenosine Triphosphate Iodoacetamide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ferrell J E
Huestis W H
References (36)
36 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.98.6.1992
Published
1984-06-00
Pages
1992-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113039
Subset
IM
Grants
NHLBI NIH HHS · HL 23787 · United States
External Links
PubMed source DOI: 10.1083/jcb.98.6.1992 PMC Full Text View journal details
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