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PMID: 6327603 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA supercoiling in gyrase mutants.

Journal of bacteriology ·Vol. 158 ·No. 2 ·1984-05-00 ·Pages 397-403

Steck TR, Pruss GJ, Manes SH, Burg L, Drlica K

Abstract

Nucleoids isolated from Escherichia coli strains carrying temperature-sensitive gyrA or gyrB mutations were examined by sedimentation in ethidium bromide-containing sucrose density gradients. A shift to restrictive temperature resulted in nucleoid DNA relaxation in all of the mutant strains. Three of these mutants exhibited reversible nucleoid relaxation: when cultures incubated at restrictive temperature were cooled to 0 degree C over a 4- to 5-min period, supercoiling returned to levels observed with cells grown at permissive temperature. Incubation of these three mutants at restrictive temperature also caused nucleoid sedimentation rates to increase by about 50%.

MeSH Terms
Centrifugation, Density Gradient DNA Topoisomerases, Type II/genetics,metabolism DNA, Bacterial/metabolism DNA, Superhelical/metabolism Escherichia coli/enzymology,genetics Mutation Temperature
Chemicals
DNA, Bacterial DNA, Superhelical DNA Topoisomerases, Type II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Steck T R
Pruss G J
Manes S H
Burg L
Drlica K
References (36)
36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.2.397-403.1984
Published
1984-05-00
Pages
397-403
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215441
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.158.2.397-403.1984 PMC Full Text View journal details
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