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PMID: 6327063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors.

Cell ·Vol. 37 ·No. 2 ·1984-06-00 ·Pages 367-79

Brinster RL, Chen HY, Messing A, van Dyke T, Levine AJ, Palmiter RD

Abstract

A high percentage of transgenic mice developing from eggs microinjected with plasmids containing the SV40 early region genes and a metallothionein fusion gene develop tumors within the choroid plexus. A line of mice has been established in which nearly every affected animal succumbs to this brain tumor. Thymic hypertrophy and kidney pathology are also observed in some mice. SV40 T-antigen mRNA and protein are readily detected in affected tissues; however, SV40 T-antigen gene expression is barely detectable in unaffected tissues or in susceptible tissues prior to overt pathology, suggesting that tumorigenesis depends upon activation of the SV40 genes. Comparison of DNA from tumor tissue (or cell lines derived from tumors) with DNA from unaffected tissues reveals structural rearrangements as well as changes in DNA methylation of the foreign DNA. The SV40 genes are frequently amplified in tumor tissue, which further indicates that their expression is intimately involved in tumorigenesis in transgenic mice.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Base Sequence Brain Neoplasms/genetics,microbiology Crosses, Genetic DNA Restriction Enzymes Female Genes Genes, Viral Male Mice Nucleic Acid Hybridization Oncogenes Pedigree Plasmids RNA, Messenger/genetics Simian virus 40/genetics Viral Proteins/genetics
Chemicals
Antigens, Polyomavirus Transforming RNA, Messenger Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Brinster R L
Chen H Y
Messing A
van Dyke T
Levine A J
Palmiter R D
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(84)90367-2
Published
1984-06-00
Pages
367-79
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC4889224
Subset
IM
Grants
NICHD NIH HHS · R01 HD017321 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(84)90367-2 PMC Full Text View journal details
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