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PMID: 6314094 Published · ppublish English Journal Article

Fine structure of transposition genes on Tn2603 and complementation of its tnpA and tnpR mutations by related transposons.

Molecular & general genetics : MGG ·Vol. 191 ·No. 3 ·1983-00-00 ·Pages 442-50

Tanaka M, Yamamoto T, Sawai T

Abstract

The fine structure of the genes tnpA, tnpR and res of Tn2603 required for its own transposition, was determined. The order of the genes was tnpA-tnpR-res from the right end of the right hand side region in Tn2603, the tnpA and tnpR encoded gene products having molecular weights of 110,000 and 21,000, respectively. The 110,000 molecular weight polypeptides was absolutely required for replicon fusion as the first stage of transposition, and named transposase. On the other hand, the 21,000 molecular weight polypeptide was necessary for resolution of the cointegrate as the second stage of transposition, and named resolvase. We also examined the ability of various transposons, assumed to be closely related, to complement the tnpA and tnpR mutations of Tn2603. The results indicated that the mercury resistance transposon, Tn2613, and Tn501, can complement both genes, but TnAs and gamma delta cannot at all. Tn501 had much less efficiency of complementation for tnpA than Tn2613. We have also discovered that the transposition frequency of transposons in the tn2613 family systematically depend on their size of transposon.

MeSH Terms
DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Genetic Complementation Test Nucleotidyltransferases/genetics Translocation, Genetic Transposases
Chemicals
DNA Transposable Elements DNA, Bacterial Nucleotidyltransferases Transposases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tanaka M
Yamamoto T
Sawai T
References (41)
41 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425761
Published
1983-00-00
Pages
442-50
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425761
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