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PMID: 6313630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane.

Journal of bacteriology ·Vol. 156 ·No. 2 ·1983-11-00 ·Pages 949-51

Kudo T, Kato C, Horikoshi K

Abstract

Two plasmids containing the penicillinase gene of alkalophilic Bacillus sp. strain 170, pEAP1 and pEAP2, were constructed. Most of the penicillinase produced by Escherichia coli, which carried these plasmids, was found in the culture medium. This excretion is caused by the cloned DNA fragment which contains some component that changes the outer membrane of E. coli.

MeSH Terms

Bacillus/enzymology,genetics DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Hydrogen-Ion Concentration Penicillinase/genetics,metabolism Plasmids Species Specificity

Chemicals

DNA Restriction Enzymes Penicillinase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kudo T

Kato C

Horikoshi K

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.156.2.949-951.1983

Published

1983-11-00

Pages

949-51

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217924

Subset

External Links

PubMed source DOI: 10.1128/jb.156.2.949-951.1983 PMC Full Text View journal details

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Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane.

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