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PMID: 6306597 Published · ppublish English Journal Article

The gene S promoter of hepatitis B virus confers constitutive gene expression.

Nucleic acids research ·Vol. 11 ·No. 13 ·1983-07-11 ·Pages 4645-54

Malpièce Y, Michel ML, Carloni G, Revel M, Tiollais P, Weissenbach J

Abstract

The properties of the promoter of the hepatitis B surface antigen (HBsAg) were studied using recombinants containing either this promoter or the SV40 early promoter. Mouse L cells were transfected with these recombinants and the levels of gene expression obtained with the two promoters were compared. The level of expression of a cellular gene, the human fibroblast interferon gene, obtained with the HBsAg promoter was comparable to that obtained with the SV40 early promoter. Similarly when the HBsAg gene was controlled by the SV40 early promoter the level of HBsAg synthesis is in the same range as that observed with its own promoter. Together these results suggest that although the HBsAg gene codes for a structural viral protein, its expression is constitutive as for an early gene. The implications of these observations on the synthesis of HBV particles in vivo are discussed.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes DNA, Recombinant/analysis Genes Genes, Viral Hepatitis B Surface Antigens/genetics Hepatitis B virus/genetics,immunology Humans Interferon Type I/genetics L Cells/metabolism Mice Operon Simian virus 40/genetics
Chemicals
DNA, Recombinant Hepatitis B Surface Antigens Interferon Type I DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Malpièce Y
Michel M L
Carloni G
Revel M
Tiollais P
Weissenbach J
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.13.4645
Published
1983-07-11
Pages
4645-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326071
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.13.4645 PMC Full Text View journal details
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