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PMID: 6957856 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulated expression of human interferon beta 1 gene after transduction into cultured mouse and rabbit cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5166-70

Canaani D, Berg P

Abstract

The human interferon beta 1 gene has been inserted into simian virus 40 hybrid plasmid vectors carrying the bacterial phosphotransferase gene (neo), and introduced into cultured mammalian cells by DNA transfection. A majority of the transformants resistant to the antibiotic G418 were capable of synthesizing and secreting biologically active human interferon. The neo/interferon transformants contain several copies of the transfecting DNA integrated into cellular DNA sequences. In most transformants the production of human interferon and its mRNA is induced by the addition of poly(rI) X poly(rC); by contrast, the level of neo mRNA is not increased under the same conditions. The 5' end of the human interferon mRNA produced after induction was indistinguishable from the interferon mRNA induced in human fibroblasts. This indicates that information enabling human beta 1 interferon gene to be induced by poly(rI) X poly(rC) is localized to sequences within, or 5'-proximal to, the coding sequence.

MeSH Terms
Animals Cells, Cultured Cloning, Molecular DNA, Superhelical/genetics Fibroblasts/metabolism Genes Humans Interferon Type I/genetics Kidney/metabolism L Cells/metabolism Male Mice Plasmids RNA, Messenger/genetics Rabbits Skin/metabolism Transduction, Genetic
Chemicals
DNA, Superhelical Interferon Type I RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Canaani D
Berg P
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.17.5166
Published
1982-09-00
Pages
5166-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346855
Subset
IM
Grants
NIGMS NIH HHS · GM-13235 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.17.5166 PMC Full Text View journal details
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