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PMID: 6304627 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The expression of prokaryotic tRNA genes in frog oocytes.

Nucleic acids research ·Vol. 11 ·No. 10 ·1983-05-25 ·Pages 3207-26

Bossi L, Ciampi MS

Abstract

A tRNA gene cluster in Salmonella typhimurium includes the genes for tRNAArg, tRNAHis, tRNA1Leu and tRNAPro. DNA clones were constructed with different portions of this tRNA gene cluster. These clones were microinjected into the nuclei of Xenopus laevis oocytes and assayed for expression. Two of the bacterial tRNA genes (tRNAArg and tRNAPro) are transcribed at high rates and the primary transcripts are processed into mature tRNAs. Transcription and processing are largely independent of whether the two genes are injected individually or as part of a tRNA gene cluster. A third tRNA gene (tRNA1Leu) is expressed less efficiently. Synthesis of this tRNA is totally abolished by a deletion removing 22 bp in the first half of the tRNA1Leu coding sequence. The expression of the fourth tRNA gene (tRNAHis) is very inefficient and dependent upon the gene organization within the injected DNA. No significant tRNA synthesis is detected upon injection of a clone containing only the tRNAHis gene. Evidence is presented suggesting that the impaired expression of the tRNAHis gene is not caused by inefficient transcription, but rather by defective processing of the primary transcript. The prokaryotic tRNAs synthesized in the oocytes show a modification pattern that is specific of eukaryotic tRNAs. Overall, our results are consistent with the hypothesis that the intragenic signals for eukaryotic tRNA gene transcription have appeared early in evolution for reasons other than gene expression.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes Female Genes Nucleic Acid Hybridization Oocytes/metabolism Ovum/metabolism Protein Biosynthesis RNA, Transfer/genetics Salmonella typhimurium/genetics Xenopus
Chemicals
RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bossi L
Ciampi M S
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.10.3207
Published
1983-05-25
Pages
3207-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325958
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.10.3207 PMC Full Text View journal details
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