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PMID: 6300760 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multiple heterogeneities in the transcribed spacers of ribosomal DNA from Xenopus laevis.

Nucleic acids research ·Vol. 11 ·No. 3 ·1983-02-11 ·Pages 629-46

Stewart MA, Hall LM, Maden BE

Abstract

Ribosomal DNA (rDNA) from Xenopus laevis contains several heterogeneities in all three transcribed spacers, as revealed by analysis of cloned and uncloned amplified rDNA from oocytes and cloned chromosomal rDNA from erythrocytes. Heterogeneities include single base changes and length variants of one to several nucleotides. Sites of variation are widely but non-uniformly distributed, some occurring only a short distance outside the boundaries of the rRNA coding regions. No two transcription units that we have yet examined are identical throughout their transcribed spacer regions.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Erythrocytes/metabolism Female Oocytes/metabolism Ovum/metabolism Transcription, Genetic Xenopus
Chemicals
DNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stewart M A
Hall L M
Maden B E
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.3.629
Published
1983-02-11
Pages
629-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325742
Subset
IM
Databases
GENBANK
K01370, K01371, K01372
External Links
PubMed source DOI: 10.1093/nar/11.3.629 PMC Full Text View journal details
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