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PMID: 6294329 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Coronavirus proteins: biogenesis of avian infectious bronchitis virus virion proteins.

Journal of virology ·Vol. 44 ·No. 3 ·1982-12-00 ·Pages 794-803

Stern DF, Sefton BM

Abstract

We examined the synthesis of viral structural proteins in cultured cells infected with the avian coronavirus infectious bronchitis virus. Tryptic peptide mapping was used to determine the structural relationships of the intracellular proteins to the virion polypeptides. Pulse-chase experiments were performed to identify precursors to the virus-specific proteins. We found that the nucleocapsid protein, P51, and the small viral membrane proteins GP31, GP28, and P23 do not undergo post-translational proteolytic processing. In contrast, GP90 and GP84, the two large virion membrane proteins, were found to be produced by cleavage of a single precursor, GP155. This demonstrated that at least one coronavirus mRNA specifies two virion proteins.

MeSH Terms
Animals Cell Line Chick Embryo Coronaviridae/genetics Electrophoresis, Polyacrylamide Gel Infectious bronchitis virus/genetics Kidney Molecular Weight Peptide Fragments/analysis Protein Biosynthesis RNA, Messenger/genetics Trypsin Viral Proteins/genetics,isolation & purification
Chemicals
Peptide Fragments RNA, Messenger Viral Proteins Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stern D F
Sefton B M
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.44.3.794-803.1982
Published
1982-12-00
Pages
794-803
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256336
Subset
IM
Grants
NCI NIH HHS · CA 14195 · United States
NCI NIH HHS · CA 17289 · United States
NIGMS NIH HHS · GM 07313 · United States
External Links
PubMed source DOI: 10.1128/JVI.44.3.794-803.1982 PMC Full Text View journal details
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