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PMID: 6294055 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mapping of chromosomal IS5 elements that mediate type II F-prime plasmid excision in Escherichia coli K-12.

Journal of bacteriology ·Vol. 153 ·No. 1 ·1983-01-00 ·Pages 395-407

Timmons MS, Bogardus AM, Deonier RC

Abstract

Three IS5 elements were mapped in overlapping chromosomal segments on a series of F-prime plasmids by restriction analysis and hybridization. IS5A was located clockwise of proA near 6 min, IS5B was located clockwise of purE near 12 min, and IS5C was tentatively located near 14 min on the Escherichia coli K-12 map. The physical structures of nine type II F-prime plasmids that contain chromosomal DNA from this region indicated that these plasmids were excised from the chromosome by recombination between pairs of IS5 elements.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Bacterial DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial Escherichia coli/genetics F Factor Nucleic Acid Hybridization Recombination, Genetic
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Timmons M S
Bogardus A M
Deonier R C
References (43)
43 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.1.395-407.1983
Published
1983-01-00
Pages
395-407
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217386
Subset
IM
Grants
NIGMS NIH HHS · GM24589 · United States
External Links
PubMed source DOI: 10.1128/jb.153.1.395-407.1983 PMC Full Text View journal details
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