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PMID: 6268939 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mapping of IS1 elements flanking the argF gene region on the Escherichia coli K-12 chromosome.

Molecular & general genetics : MGG ·Vol. 181 ·No. 2 ·1981-00-00 ·Pages 222-9

Hu M, Deonier RC

Abstract

Two directly-repeated IS1 elements have been mapped on the Escherichia coli K-12 chromosome at positions 23.2 kb and 34.5 kb counterclockwise of the IS3 element alpha3beta3 by using F-prime plasmids (including the F lac- proAB+ plasmid F128) that carry different portions of the bacterial chromosome in the purE to proA region. Mapping was accomplished in part by construction of EcoRI, BamHI, and BglII restriction enzyme cleavage maps. Electron microscope heteroduplex and hybridization studies indicate that the chromosomal region flanked by these IS1 elements is completely homologous to the IS1-argF-IS1 region (Tn2901) on the P1argF5 transducing phage (York and Stodolsky, 1981), which suggests that the argF gene region in the usual E. coli K-12 strains has a transposon-like structure.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Coliphages/genetics DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial Escherichia coli/genetics F Factor Microscopy, Electron Nucleic Acid Hybridization Ornithine Carbamoyltransferase/genetics Plasmids Transduction, Genetic
Chemicals
DNA Transposable Elements DNA, Bacterial Ornithine Carbamoyltransferase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hu M
Deonier R C
References (34)
34 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268430
Published
1981-00-00
Pages
222-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM-24589 · United States
External Links
PubMed source DOI: 10.1007/BF00268430
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