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PMID: 6292451 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of tunicamycin on rotavirus assembly and infectivity.

Journal of virology ·Vol. 43 ·No. 3 ·1982-09-00 ·Pages 1082-90

Sabara M, Babiuk LA, Gilchrist J, Misra V

Abstract

Bovine rotavirus grown in the presence or absence of tunicamycin was analyzed with respect to yield of infectious virus, the ratio of complete to incomplete particles, and polypeptide composition. Tunicamycin at a concentration of 1 microgram/ml reduced virus yields by 4 logs and completely prevented the incorporation of [3H]uridine into complete rotavirus particles, as determined by cesium chloride gradient analysis. Concomitant with a reduction in complete particles, three rotavirus polypeptides shifted in their relative position on polyacrylamide gels from 41,900-molecular-weight position (41.9K), 29.3K, and 16.1K to migrate at 35.5K, 22.7K, and 15.5K, respectively. Limited proteolysis indicated that the lower-molecular-weight polypeptides possessed the same constituent peptides as the larger polypeptides, suggesting that they represented the unglycosylated equivalents. These results suggest that interference with glycosylation prevents proper assembly of the outer coat proteins in bovine rotavirus.

MeSH Terms
Animals Capsid/metabolism Cattle Glucosamine/analogs & derivatives Glycoproteins/analysis Morphogenesis/drug effects Peptides/analysis Rotavirus/drug effects,pathogenicity,ultrastructure Tunicamycin/pharmacology Viral Proteins/analysis Virulence/drug effects
Chemicals
Glycoproteins Peptides Viral Proteins Tunicamycin Glucosamine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sabara M
Babiuk L A
Gilchrist J
Misra V
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29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.3.1082-1090.1982
Published
1982-09-00
Pages
1082-90
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256220
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.43.3.1082-1090.1982 PMC Full Text View journal details
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