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PMID: 691111 Published · ppublish English Journal Article

Adsorption and penetration of enveloped and naked vaccinia virus particles.

Journal of virology ·Vol. 27 ·No. 1 ·1978-07-00 ·Pages 19-27

Payne LG, Norrby E

Abstract

The adsorption and penetration of intracellular naked vaccinia virus (INV) and extracellular enveloped vaccinia virus (EEV) were examined. The adsorption kinetics of INV and EEV were similar, but INV adsorption was found to be more sensitive to the adsorption environment than EEV. The PFU-to-particle ratio for the two virus particles indicated that EEV was approximately two times as infectious as INV. Kinetic studies at 37 degree C showed that EEV penetrated cells more rapidly than INV. Penetration of EEV was unaffected by incubation in phsophate-buffered saline, but was somewhat reduced by incubation at 22 degree C. In contrast, INV penetration was effectively eliminated by incubation in phosphate-buffered saline or by incubation at 22 degree C. In addition, INV but not EEV pentration was sensitive to treatment with sodium fluoride and cytochalasin. B. These results are discussed with regard to the mechanism of INV and EEV penetration.

MeSH Terms
Adsorption Cell Line Cell Membrane/microbiology Cytochalasin B/pharmacology Kinetics Sodium Fluoride/pharmacology Vaccinia virus/growth & development,metabolism Viral Plaque Assay
Chemicals
Cytochalasin B Sodium Fluoride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Payne L G
Norrby E
References (28)
28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.27.1.19-27.1978
Published
1978-07-00
Pages
19-27
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354136
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.27.1.19-27.1978 PMC Full Text View journal details
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