Solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus antibody.
An indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. The test involved ethanol fixation of microcultures of bovine rotavirus-infected BSC-1 cells and reacetion with bovine antirotavirus serum, followed by 125I-labeled rabbit anti-bovine immunoglobulin G. The technique was shown to be virus specific and highly sensitive. The fixed microcultures could be stored at 4 degrees C for at least 2 months without affecting the sensitivity of the test. The application of this system for the detection of rotavirus antibodies in humans is briefly discussed.
17 references, click to expand
-
Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody.J Clin Pathol. 1976 Mar;29(3):191-7 PMID: 1270600
-
Viral enteritis of calves.Vet Rec. 1975 Jan 25;96(4):85-8 PMID: 163517
-
Detection by radioimmunoassay of antibodies in human smallpox patients and vaccinees.J Clin Microbiol. 1975 Mar;1(3):311-7 PMID: 170309
-
Solid phase radioimmunoassay for typing herpes simplex viral antibodies in human sera.J Clin Microbiol. 1975 Nov;2(5):410-8 PMID: 172526
-
Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test.J Clin Microbiol. 1976 Mar;3(3):376-7 PMID: 178691
-
Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis.Lancet. 1973 Dec 8;2(7841):1281-3 PMID: 4127639
-
Labeled staphylococcal protein A as an immunological probe in the analysis of cell surface markers.Scand J Immunol. 1974;3(4):405-11 PMID: 4136845
-
Relation between viruses from acute gastroenteritis of children and newborn calves.Lancet. 1974 Jul 13;2(7872):61-3 PMID: 4137164
-
Cell culture adaptation and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in Nebraska.Arch Gesamte Virusforsch. 1972;37(1):114-30 PMID: 4623479
-
Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125 I-antibody.J Immunol. 1972 Oct;109(4):834-41 PMID: 4627513
-
Rapid quantitation of membrane antigens.Nature. 1975 Mar 6;254(5495):67-9 PMID: 46592
-
Further observations on the virus of epizootic diarrhea of infant mice. An electron microscopic study.Virology. 1968 Nov;36(3):411-21 PMID: 4972583
-
Assay of Australia antigen and antibody employing double-antibody and solid-phase radioimmunoassay techniques and comparison with the passive hemagglutination methods.J Immunol. 1971 Oct;107(4):1099-111 PMID: 5093775
-
Defense mechanisms against bovine herpesvirus: relationship of virus-host cell events to susceptibility to antibody-complement cell lysis.Infect Immun. 1975 Nov;12(5):958-63 PMID: 53206
-
Antigenic relationships among five reovirus-like (RVL) agents by complement fixation (CF) and development of new substitute CF antigens for the human RVL agent of infantile gastroenteritis.Proc Soc Exp Biol Med. 1976 Sep;152(4):535-9 PMID: 61589
-
Reovirus-like agent (rotavirus) from lambs.Infect Immun. 1976 Dec;14(6):1332-8 PMID: 793991
-
Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals.Infect Immun. 1976 Sep;14(3):804-10 PMID: 965097
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
