Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody.

Journal of clinical pathology ·Vol. 29 ·No. 3 ·1976-03-00 ·Pages 191-7

Middleton PJ, Petric M, Hewitt CM, Szymanski MT, Tam JS

Abstract

A moderatley sensitive, rapid, and economical test scheme for the detection of infantile gastroenteritis virus (IGV) in stool or antibody in serum has been developed and evaluated. The test scheme with minor modifications was an adaptation of a counter-immunoelectro-osmophoresis system we once used for the detection of hepatitis B antigen. Large numbers of stool samples may be screened during half a working day for the presence of IGV using reference antiserum to IGV prepared in guinea-pigs. Serological studies of a diagnostic but not epidemiological nature may also be performed with equal facility by this same test scheme using highly purified IGV antigen derived from stool.

MeSH Terms

Adolescent Adult Animals Antibodies, Viral/analysis Antigens, Viral/analysis Cattle Child Child, Preschool Complement Fixation Tests Counterimmunoelectrophoresis Evaluation Studies as Topic Feces/microbiology Gastroenteritis/immunology,microbiology Guinea Pigs Humans Immunoelectrophoresis Infant Infant, Newborn Viruses/isolation & purification

Chemicals

Antibodies, Viral Antigens, Viral

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Middleton P J

Petric M

Hewitt C M

Szymanski M T

Tam J S

References (13)

13 references, click to expand

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Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.29.3.191

Published

1976-03-00

Pages

191-7

Language

English

Region

England

NLM ID

0376601

PMCID

PMC476020

Subset

External Links

PubMed source DOI: 10.1136/jcp.29.3.191 PMC Full Text View journal details

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Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody.

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