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PMID: 6290455 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The incompatibility product of IncFII R plasmid NR1 controls gene expression in the plasmid replication region.

Journal of bacteriology ·Vol. 152 ·No. 2 ·1982-11-00 ·Pages 829-39

Easton AM, Rownd RH

Abstract

The incompatibility properties of IncFII R plasmid NR1 were compared with those of two of its copy number mutants, pRR12 and pRR21. pRR12 produced an altered incompatibility product and also had an altered incompatibility target site. The target site appeared to be located within the incompatibility gene, which is located more than 1,200 base pairs from the plasmid origin of replication. The incompatibility properties of pRR21 were indistinguishable from those of NR1. Lambda phages have been constructed which contain the incompatibility region of NR1 or of one of its copy mutants fused to the lacZ gene. In lysogens constructed with these phages, beta-galactosidase was produced under the control of a promoter located within the plasmid incompatibility region. Lysogens containing prophages with the incompatibility regions from pRR12 and pRR21 produced higher levels of beta-galactosidase than did lysogens containing prophages with the incompatibility region from the wild-type NR1. The introduction into these inc-lac lysogens of pBR322 plasmids carrying the incompatibility regions of the wild-type or mutant plasmids resulted in decreased levels of beta-galactosidase production. For a given lysogen, the decrease was greater when the pBR322 derivative expressed a stronger incompatibility toward the plasmid from which the fragment in the prophage was derived. This suggested that the incompatibility product acts on its target to repress gene expression in the plasmid replication region.

MeSH Terms
Bacteriophage lambda/genetics DNA Replication DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/enzymology,genetics Genes Lysogeny Mutation Nucleic Acid Hybridization Plasmids beta-Galactosidase/genetics
Chemicals
DNA, Recombinant DNA Restriction Enzymes beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Easton A M
Rownd R H
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.2.829-839.1982
Published
1982-11-00
Pages
829-39
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221537
Subset
IM
Grants
NIGMS NIH HHS · GM 14398 · United States
External Links
PubMed source DOI: 10.1128/jb.152.2.829-839.1982 PMC Full Text View journal details
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