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PMID: 6287456 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Proteins specified by avian erythroblastosis virus: coding region localization and identification of a previously undetected erb-B polypeptide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 13 ·1982-07-00 ·Pages 3958-62

Privalsky ML, Bishop JM

Abstract

Avian erythroblastosis virus (AEV) induces erythroblastosis and sarcomas in chickens. Two domains within the viral genome, erb-A and erb-B, have been implicated in AEV-mediated oncogenesis. By use of hybridization-arrested translations and hybridization-selections of mRNA, we have mapped on the viral genome the polypeptides specified by the erb domains. The results of hybridization-arrest with DNA representing the spliced 5' leader region of the AEV mRNA suggested that the authentic product of the erb-B domain was a 61,000 molecular weight protein, not a 41,000 molecular weight polypeptide previously identified.

MeSH Terms
Alpharetrovirus/genetics Animals Avian Leukosis Virus/genetics Cell Transformation, Viral Chickens Erythrocytes Nucleic Acid Hybridization Peptides/genetics Plasmids Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics
Chemicals
Peptides RNA, Messenger RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Privalsky M L
Bishop J M
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.13.3958
Published
1982-07-00
Pages
3958-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346555
Subset
IM
Grants
NCI NIH HHS · 08T2 CA 09043 · United States
NCI NIH HHS · CA 12705 · United States
NCI NIH HHS · CA 19287 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.13.3958 PMC Full Text View journal details
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